Pun, Suzie H. and Bellocq, Nathalie C. and Liu, Aijie and Jensen, Greg and Machemer, Todd and Quijano, Erlinda and Schluep, Thomas and Wen, Shufen and Engler, Heidrun and Heidel, Jeremy and Davis, Mark E. (2004) Cyclodextrin-Modified Polyethylenimine Polymers for Gene Delivery. Bioconjugate Chemistry, 15 (4). pp. 831-840. ISSN 1043-1802. doi:10.1021/bc049891g. https://resolver.caltech.edu/CaltechAUTHORS:20170125-093852180
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Abstract
Linear and branched poly(ethylenimines), lPEI and bPEI, respectively, grafted with β-cyclodextrin are prepared to give CD−lPEI and CD−bPEI, respectively, and are investigated as in vitro and in vivo nonviral gene delivery agents. The in vitro toxicity and transfection efficiency are sensitive to the level of cyclodextrin grafting. The cyclodextrin-containing polycations, when combined with adamantane−poly(ethylene glycol) (AD−PEG) conjugates, form particles that are stable at physiological salt concentrations. PEGylated CD−lPEI-based particles give in vitro gene expression equal to or greater than lPEI as measured by the percentage of EGFP expressing cells. Tail vein injections into mice of 120 μg of plasmid DNA formulated with CD−lPEI and AD−PEG do not reveal observable toxicities, and both nucleic acid accumulation and expression are observed in liver.
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Additional Information: | © 2004 American Chemical Society. Received 3 May 2004; Published online 29 June 2004; Published in print 1 July 2004. J.D.H. acknowledges the Whitaker Foundation for a doctoral fellowship. | ||||||
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Issue or Number: | 4 | ||||||
DOI: | 10.1021/bc049891g | ||||||
Record Number: | CaltechAUTHORS:20170125-093852180 | ||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20170125-093852180 | ||||||
Official Citation: | Cyclodextrin-Modified Polyethylenimine Polymers for Gene Delivery Suzie H. Pun, Nathalie C. Bellocq, Aijie Liu, Greg Jensen, Todd Machemer, Erlinda Quijano, Thomas Schluep, Shufen Wen, Heidrun Engler, Jeremy Heidel, and Mark E. Davis Bioconjugate Chemistry 2004 15 (4), 831-840 DOI: 10.1021/bc049891g | ||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||
ID Code: | 73707 | ||||||
Collection: | CaltechAUTHORS | ||||||
Deposited By: | Ruth Sustaita | ||||||
Deposited On: | 25 Jan 2017 19:02 | ||||||
Last Modified: | 11 Nov 2021 05:20 |
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