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Cyclodextrin-Modified Polyethylenimine Polymers for Gene Delivery

Pun, Suzie H. and Bellocq, Nathalie C. and Liu, Aijie and Jensen, Greg and Machemer, Todd and Quijano, Erlinda and Schluep, Thomas and Wen, Shufen and Engler, Heidrun and Heidel, Jeremy and Davis, Mark E. (2004) Cyclodextrin-Modified Polyethylenimine Polymers for Gene Delivery. Bioconjugate Chemistry, 15 (4). pp. 831-840. ISSN 1043-1802. doi:10.1021/bc049891g. https://resolver.caltech.edu/CaltechAUTHORS:20170125-093852180

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Abstract

Linear and branched poly(ethylenimines), lPEI and bPEI, respectively, grafted with β-cyclodextrin are prepared to give CD−lPEI and CD−bPEI, respectively, and are investigated as in vitro and in vivo nonviral gene delivery agents. The in vitro toxicity and transfection efficiency are sensitive to the level of cyclodextrin grafting. The cyclodextrin-containing polycations, when combined with adamantane−poly(ethylene glycol) (AD−PEG) conjugates, form particles that are stable at physiological salt concentrations. PEGylated CD−lPEI-based particles give in vitro gene expression equal to or greater than lPEI as measured by the percentage of EGFP expressing cells. Tail vein injections into mice of 120 μg of plasmid DNA formulated with CD−lPEI and AD−PEG do not reveal observable toxicities, and both nucleic acid accumulation and expression are observed in liver.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/bc049891gDOIArticle
ORCID:
AuthorORCID
Pun, Suzie H.0000-0003-1443-4996
Davis, Mark E.0000-0001-8294-1477
Additional Information:© 2004 American Chemical Society. Received 3 May 2004; Published online 29 June 2004; Published in print 1 July 2004. J.D.H. acknowledges the Whitaker Foundation for a doctoral fellowship.
Funders:
Funding AgencyGrant Number
Whitaker FoundationUNSPECIFIED
Issue or Number:4
DOI:10.1021/bc049891g
Record Number:CaltechAUTHORS:20170125-093852180
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170125-093852180
Official Citation:Cyclodextrin-Modified Polyethylenimine Polymers for Gene Delivery Suzie H. Pun, Nathalie C. Bellocq, Aijie Liu, Greg Jensen, Todd Machemer, Erlinda Quijano, Thomas Schluep, Shufen Wen, Heidrun Engler, Jeremy Heidel, and Mark E. Davis Bioconjugate Chemistry 2004 15 (4), 831-840 DOI: 10.1021/bc049891g
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:73707
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:25 Jan 2017 19:02
Last Modified:11 Nov 2021 05:20

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