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Synthetic Biocompatible Cyclodextrin-Based Constructs for Local Gene Delivery to Improve Cutaneous Wound Healing

Bellocq, Nathalie C. and Kang, David W. and Wang, Xuehui and Jensen, Gregory S. and Pun, Suzie H. and Schluep, Thomas and Zepeda, Monica L. and Davis, Mark E. (2004) Synthetic Biocompatible Cyclodextrin-Based Constructs for Local Gene Delivery to Improve Cutaneous Wound Healing. Bioconjugate Chemistry, 15 (6). pp. 1201-1211. ISSN 1043-1802. doi:10.1021/bc0498119. https://resolver.caltech.edu/CaltechAUTHORS:20170125-115431256

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Abstract

The localized, sustained delivery of growth factors for wound healing therapy is actively being explored by gene transfer to the wound site. Biocompatible matrices such as bovine collagen have demonstrated usefulness in sustaining gene therapy vectors that express growth factors in local sites for tissue repair. Here, new synthetic biocompatible materials are prepared and shown to deliver a protein to cultured cells via the use of an adenoviral delivery vector. The synthetic construct consists of a linear, β-cyclodextrin-containing polymer and an adamantane-based cross-linking polymer. When the two polymers are combined, they create an extended network by the formation of inclusion complexes between the cyclodextrins and adamantanes. The properties of the network are altered by controlling the polymer molecular weights and the number of adamantanes on the cross-linking polymer, and these modifications and others such as replacement of the β-cyclodextrin (host) and adamantane (guest) with other cyclodextrins (hosts such as α, γ, and substituted members) and inclusion complex forming molecules (guests) provide the ability to rationally design network characteristics. Fibroblasts exposed to these synthetic constructs show proliferation rates and migration patterns similar to those obtained with collagen. Gene delivery (green fluorescent protein) to fibroblasts via the inclusion of adenoviral vectors in the synthetic construct is equivalent to levels observed with collagen. These in vitro results suggest that the synthetic constructs are suitable for in vivo tissue repair applications.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/bc0498119DOIArticle
ORCID:
AuthorORCID
Pun, Suzie H.0000-0003-1443-4996
Davis, Mark E.0000-0001-8294-1477
Additional Information:© 2004 American Chemical Society. Received 2 August 2004. Published online 26 October 2004. Published in print 1 November 2004.
Issue or Number:6
DOI:10.1021/bc0498119
Record Number:CaltechAUTHORS:20170125-115431256
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170125-115431256
Official Citation:Synthetic Biocompatible Cyclodextrin-Based Constructs for Local Gene Delivery to Improve Cutaneous Wound Healing Nathalie C. Bellocq, David W. Kang, Xuehui Wang, Gregory S. Jensen, Suzie H. Pun, Thomas Schluep, Monica L. Zepeda, and Mark E. Davis Bioconjugate Chemistry 2004 15 (6), 1201-1211 DOI: 10.1021/bc0498119
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:73720
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:25 Jan 2017 23:44
Last Modified:11 Nov 2021 05:21

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