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Kinetic Model for the ATP-Dependent Translocation of Saccharomyces cerevisiae RSC along Double-Stranded DNA

Fischer, Christopher J. and Saha, Anjanabha and Cairns, Bradley R. (2007) Kinetic Model for the ATP-Dependent Translocation of Saccharomyces cerevisiae RSC along Double-Stranded DNA. Biochemistry, 46 (43). pp. 12416-12426. ISSN 0006-2960. PMCID PMC2810488. https://resolver.caltech.edu/CaltechAUTHORS:20170201-074622359

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Abstract

The chromatin remodeling complex RSC from Saccharomyces cerevisiae is a DNA translocase that moves with directionality along double-stranded DNA in a reaction that is coupled to ATP hydrolysis. To better understand how this basic molecular motor functions, a novel method of analysis has been developed to study the kinetics of RSC translocation along double-stranded DNA. The data provided are consistent with RSC translocation occurring through a series of repeating uniform steps with an overall processivity of P = 0.949 ± 0.003; this processivity corresponds to an average translocation distance of 20 ± 1 base pairs (bp) before dissociation. Interestingly, a slow initiation process, following DNA binding, is required to make RSC competent for DNA translocation. These results are further discussed in the context of previously published studies of RSC and other DNA translocases.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/bi700930nDOIArticle
http://pubs.acs.org/doi/full/10.1021/bi700930nPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2810488/PubMed CentralArticle
ORCID:
AuthorORCID
Cairns, Bradley R.0000-0002-9864-8811
Additional Information:© 2007 American Chemical Society. Received 15 May 2007. Published online 5 October 2007. Published in print 1 October 2007. This research was supported, in part, by startup funding from the University of Kansas (to C.J.F.) and by the National Institutes of Health (GM60415 to B.R.C., support of A.S.). B.R.C. is an Investigator with the Howard Hughes Medical Institute. We thank the University of Kansas Department Molecular Biosciences and especially Mark Richter for the use of common equipment. In addition, we thank the University of Kansas Department of Pharmacology and Toxicology, especially Dr. Abdulbaki Agbas and Dr. Elias K. Michaelis, for the use of the department's phosphorimager. We thank Janet Lindsley (Department of Biochemistry, University of Utah) for generously allowing access to stopped-flow equipment and for expert advice on conducting the experiments. We thank Bob Schackmann for oligonucleotide synthesis (Core Facilities).
Funders:
Funding AgencyGrant Number
University of KansasUNSPECIFIED
NIHGM60415
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Issue or Number:43
PubMed Central ID:PMC2810488
Record Number:CaltechAUTHORS:20170201-074622359
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170201-074622359
Official Citation:Kinetic Model for the ATP-Dependent Translocation of Saccharomyces cerevisiae RSC along Double-Stranded DNA Christopher J. Fischer, Anjanabha Saha, and Bradley R. Cairns Biochemistry 2007 46 (43), 12416-12426 DOI: 10.1021/bi700930n
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:73905
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:01 Feb 2017 16:57
Last Modified:09 Mar 2020 13:18

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