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Development and experimental validation of a mechanistic model of a recombinase-based temporal logic gate

Bowyer, Jack and Hsiao, Victoria and Bates, Declan G. (2016) Development and experimental validation of a mechanistic model of a recombinase-based temporal logic gate. In: 2016 IEEE Biomedical Circuits and Systems Conference (BioCAS). IEEE , Piscataway, NJ, pp. 464-467. ISBN 978-1-5090-2959-4 . http://resolver.caltech.edu/CaltechAUTHORS:20170201-172258387

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Abstract

DNA recombination provides an ideal mechanism for constructing stable and reversible synthetic biological switches. Recent advances in recombinase-based circuitry that account for more than one protein input have been shown to enable the construction of circuits with temporal Boolean logic operations in vivo. Associated mathematical models have to date only captured the qualitative dynamical features of such systems and are thus of limited utility as tools to aid in the design of such circuitry. Here we develop a detailed mechanistic model of a two-input temporal logic gate circuit based on unidirectional DNA recombination with bacteriophage integrases to detect and encode sequences of input events. The model is validated against in vivo experimental data and is shown to quantitatively replicate and predict key dynamical features of the logic gate.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1109/BioCAS.2016.7833832DOIArticle
http://ieeexplore.ieee.org/document/7833832/PublisherArticle
ORCID:
AuthorORCID
Hsiao, Victoria0000-0001-9297-1522
Additional Information:© 2016 IEEE. Research supported by EPSRC via a DTA studentship to J. Bowyer.
Funders:
Funding AgencyGrant Number
Engineering and Physical Sciences Research Council (EPSRC)UNSPECIFIED
Record Number:CaltechAUTHORS:20170201-172258387
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20170201-172258387
Official Citation:J. Bowyer, V. Hsiao and D. G. Bates, "Development and experimental validation of a mechanistic model of a recombinase-based temporal logic gate," 2016 IEEE Biomedical Circuits and Systems Conference (BioCAS), Shanghai, China, 2016, pp. 464-467. doi: 10.1109/BioCAS.2016.7833832
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:73972
Collection:CaltechAUTHORS
Deposited By: Kristin Buxton
Deposited On:02 Feb 2017 02:32
Last Modified:07 Feb 2017 00:53

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