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Radiation leukemia virus and X-irradiation induce in C57BL/6 mice two distinct T-cell neoplasms: A growth factor-dependent lymphoma and a growth factor-independent lymphoma

Haas, Martin and Altman, Amnon and Rothenberg, Ellen and Bogart, Mark H. and Jones, O. W. (1987) Radiation leukemia virus and X-irradiation induce in C57BL/6 mice two distinct T-cell neoplasms: A growth factor-dependent lymphoma and a growth factor-independent lymphoma. Leukemia Research, 11 (3). pp. 223-239. ISSN 0145-2126. https://resolver.caltech.edu/CaltechAUTHORS:20170206-144350445

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Abstract

Two different classes of neoplastic T cells were isolated from radiation leukemia virus (RadLV)-inoculated and from X-ray-treated C57BL/6 mice. One consisted of growth factor-dependent T-cell lymphoma (FD-TCL) lines which were established from the spleens and thymuses of treated mice within a day of lymphoma detection. FD-TCL cells were often eudiploid and could be grown in pure culture only at high concentrations, or on stromal feeder layers. Non-thymic, factor-dependent TCL cells produced interleukin-2 upon lectin stimulation, and were autostimulatory because they secreted growth factor(s) constitutively. Single cell cloning of FD-TCL cells in semisolid medium required the addition of exogenous conditioned medium. In vivo, FD-TCL cells that were injected intraperitoneally or intravenously homed to the spleen, proliferated in it and killed the injected mice. FD-TCL cells did not produce local tumors at the site of subcutaneous injection. The isolation and study of FD-TCL cells were facilitated by their cultivation on stromal hemotopoietic monolayers in supplemented “lymphocyte medium”, until an autostimulating, self-sustaining concentration of FD-TCL cells was obtained. FD-TCL cells could not be grown from lymphoid tissue of normal, control mice. In contrast, T-cell lymphoma (TCL) lines, which were established from virus-induced thymomas which had been kept in situ for 4–6 weeks after detection, consisted of factor-independent cells that possessed an aneuploid karyotype (in some cases trisomic for chromosome No. 15), and produced local tumors at the site of subcutaneous injection. These cells could be cloned in semisolid medium without addition of exogenous factor(s). The phenotypic markers of TCL cells differed from those of FD-TCL cells, suggesting heterogeneity in the stages of differentiation at which cells can give rise to growth factor-independent (TCL) and to growth factor-dependent (FD-TCL) lines.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0145-2126(87)90046-4DOIArticle
http://www.sciencedirect.com/science/article/pii/0145212687900464?via%3DihubPublisherArticle
ORCID:
AuthorORCID
Rothenberg, Ellen0000-0002-3901-347X
Additional Information:© 1987 Elsevier Ltd. Received 18 October 1983, Accepted 3 September 1986. Supported in part by grant CA 34151 from the US Public Health Service, and by Specialized Cancer Center Core Support Grant 5 P30 CA 23100.
Funders:
Funding AgencyGrant Number
NIHCA34151
NIH5 P30 CA 23100
Subject Keywords:T-cell lymphoma; lymphoma growth factor
Issue or Number:3
Record Number:CaltechAUTHORS:20170206-144350445
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170206-144350445
Official Citation:Martin Haas, Amnon Altman, Ellen Rothenberg, Mark H. Bogart, O.W. Jones, Radiation leukemia virus and X-irradiation induce in C57BL/6 mice two distinct T-cell neoplasms: A growth factor-dependent lymphoma and a growth factor-independent lymphoma, Leukemia Research, Volume 11, Issue 3, 1987, Pages 223-239, ISSN 0145-2126, http://dx.doi.org/10.1016/0145-2126(87)90046-4. (http://www.sciencedirect.com/science/article/pii/0145212687900464) Keywords: T-cell lymphoma; lymphoma growth factor
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:74097
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:06 Feb 2017 23:50
Last Modified:03 Oct 2019 16:34

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