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In vitro maintenance of differentiation marker synthesis by subpopulations of mouse thymocytes

Rothenberg, Ellen and Triglia, Dennis (1980) In vitro maintenance of differentiation marker synthesis by subpopulations of mouse thymocytes. Journal of Supramolecular Structure, 14 (3). pp. 371-382. ISSN 0091-7419. doi:10.1002/jss.400140310. https://resolver.caltech.edu/CaltechAUTHORS:20170206-150943857

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Abstract

Mouse thymocyte populations enriched in functionally incompetent, “immature” cells on the one hand, or in competent “mature” cells on the other hand, express different steady-state levels of certain surface antigens and marker enzymes. In the cases of the glycoproteins H-2 (K and D), Qa, and TL, and the DNA polymerase terminal deoxynucleotidyl transferase (TdT), these levels reflect different rates of de novo synthesis in the two populations. Thus each population appears to manifest a characteristic pattern of synthetic rates for the various products relative to total protein synthesis. To investigate the maintenance of these patterns, enriched pools of “immature” and “mature” thymocytes were incubated in vitro for 24 h, and the rates of product synthesis before and after culture were compared. H-2 synthesis, initially most rapid in the mature cells, continued to be made at the highest rate in this population. TdT synthesis, a characteristic activity of the immature cells, was not induced in the mature cells, but proceeded at an increased relative rate in the immature population. Therefore, the differences between the rates of H-2 and TdT synthesis were stable properties of the two thymocyte populations. Another marker of immature cells, TL, did not continue to be produced in parallel with TdT. Rather, its synthesis was selectively curtailed in relation to the continuing protein synthesis in the immature cultures. This non-coordinate regulation of TL and TdT production in immature thymocytes may be due to several mechanisms. These are discussed with regard to their implications for pathways of thymocyte maturation.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/jss.400140310DOIArticle
http://onlinelibrary.wiley.com/doi/10.1002/jss.400140310/abstractPublisherArticle
ORCID:
AuthorORCID
Rothenberg, Ellen0000-0002-3901-347X
Additional Information:© 1980 Alan R. Liss, Inc. Issue online: 19 February 2004; Version of record online: 19 February 2004; Manuscript Accepted: 25 August 1980; Manuscript Received: 28 April 1980.
Subject Keywords:thymocyte subpopulations; differentiation antigens; PNA fractionation; density gradient; biosynthetic labeling; short-term culture; non-coordinate regulation
Issue or Number:3
DOI:10.1002/jss.400140310
Record Number:CaltechAUTHORS:20170206-150943857
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170206-150943857
Official Citation:Rothenberg, E. and Triglia, D. (1980), In vitro maintenance of differentiation marker synthesis by subpopulations of mouse thymocytes. J. Supramol. Struct., 14: 371–382. doi:10.1002/jss.400140310
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:74103
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:06 Feb 2017 23:36
Last Modified:11 Nov 2021 05:24

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