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Developmental and Molecular Characterization of Emerging β- and γδ-Selected Pre-T Cells in the Adult Mouse Thymus

Taghon, Tom and Yui, Mary A. and Pant, Rashmi and Diamond, Rochelle A. and Rothenberg, Ellen V. (2006) Developmental and Molecular Characterization of Emerging β- and γδ-Selected Pre-T Cells in the Adult Mouse Thymus. Immunity, 24 (1). pp. 53-64. ISSN 1074-7613. https://resolver.caltech.edu/CaltechAUTHORS:20170207-080106709

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Abstract

The first checkpoint in T cell development, β selection, has remained incompletely characterized for lack of specific surface markers. We show that CD27 is upregulated in DN3 thymocytes initiating β selection, concomitant with intracellular TCR-β expression. Clonal analysis determined that CD27^(high) DN3 cells generate CD4^+CD8^+ progeny with more than 90% efficiency, faster and more efficiently than the CD27^(low) majority. CD27 upregulation also occurs in γδ-selected DN3 thymocytes in TCR-β−/− mice and in IL2-GFP transgenic reporter mice where GFP marks the earliest emerging TCR-γδ cells from DN3 thymocytes. With CD27 to distinguish pre- and postselection DN3 cells, a detailed gene expression analysis defined regulatory changes associated with checkpoint arrest, with β selection, and with γδ selection. γδ selection induces higher CD5, Egr, and Runx3 expression as compared to β selection, but it triggers less proliferation. Our results also reveal differences in Notch/Delta dependence at the earliest stages of divergence between developing αβ and γδ T-lineage cells.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.immuni.2005.11.012DOIArticle
http://www.sciencedirect.com/science/article/pii/S1074761305004073PublisherArticle
ORCID:
AuthorORCID
Yui, Mary A.0000-0002-3136-2181
Rothenberg, Ellen V.0000-0002-3901-347X
Additional Information:© 2006 Elsevier Inc. Received 4 August 2005, Revised 6 November 2005, Accepted 30 November 2005, Available online 17 January 2006. Published: January 17, 2006. We thank Michele K. Anderson and Elizabeth-Sharon David for primers; Ruben Bayon for animal care; and Stephanie Adams and Pat Koen of the Caltech Flow Cytometry/Cell Sorting Facility. We apologize to colleagues whose work we could not cite. This work was supported by grants from the NIH (R01 CA98925 and R01 CA90233).
Funders:
Funding AgencyGrant Number
NIHR01 CA98925
NIHR01 CA90233
Issue or Number:1
Record Number:CaltechAUTHORS:20170207-080106709
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170207-080106709
Official Citation:Tom Taghon, Mary A. Yui, Rashmi Pant, Rochelle A. Diamond, Ellen V. Rothenberg, Developmental and Molecular Characterization of Emerging β- and γδ-Selected Pre-T Cells in the Adult Mouse Thymus, Immunity, Volume 24, Issue 1, January 2006, Pages 53-64, ISSN 1074-7613, http://dx.doi.org/10.1016/j.immuni.2005.11.012. (//www.sciencedirect.com/science/article/pii/S1074761305004073)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:74126
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:07 Feb 2017 16:07
Last Modified:03 Oct 2019 16:35

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