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A specific biosynthetic marker for immature thymic lymphoblasts. Active synthesis of thymus-leukemia antigen restricted to proliferating cells

Rothenberg, Ellen (1982) A specific biosynthetic marker for immature thymic lymphoblasts. Active synthesis of thymus-leukemia antigen restricted to proliferating cells. Journal of Experimental Medicine, 155 (1). pp. 140-154. ISSN 0022-1007. PMCID PMC2186575. https://resolver.caltech.edu/CaltechAUTHORS:20170207-084442559

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Abstract

Large cortical thymocytes from C57BL/6-Tla(a) mice have been prepared rapidly and in high yield by a combination of centrifugal elutriation and differential binding to peanut agglutinin (PNA)-coated plates. The cells in these lymphoblast-rich fractions were clearly distinct from the majority of thymocytes, with up to 70 percent in the S or G(2) + M phases of the cell cycle and an average rate of [(35)S]methionine incorporation per cell up to 20 times higher than that of the majority population. The populations of cells resolved in this fractionation were characterized by monitoring their rates of synthesis of specific glycoproteins, thymus- leukemia antigen (TL) and the Lyt-2, Lyt-3 complex (Lyt-2/3), relative to their total protein synthesis. Cells that bound to PNA synthesized high levels of Lyt-2/3, consistent with their identification as cortical thymocytes. Those that failed to bind made little or no Lyt-2/3, as expected for medullary cells, The fraction of dividing lymphoblasts that bound to PNA was enriched in cortical thymocyte precursors, including all the large cells detectably active in synthesizing Lyt-2. It differed sharply from the small cortical cells, however, in the synthesis of TL. Although both populations displayed abundant surface TL, the TL glycoprotein was produced actively in fractions containing dividing cells but made at a drastically reduced rate by the nondividing majority of cortical thymocytes. Thus, TL seems to be made at a narrowly circumscribed stage of early thymocyte development that is correlated with rapid proliferation. In most of the descendants of such blast cells, the TL glycoprotein is presumably retained on the cell surface as long as no substantial membrane turnover takes place. Ongoing TL synthesis may therefore serve as a marker for a unique developmental state which terminates rapidly in normal differentiation but may be extended by agents that give rise to TL(+) thymic lymphomas.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1084/jem.155.1.140DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2186575/PubMed CentralArticle
ORCID:
AuthorORCID
Rothenberg, Ellen0000-0002-3901-347X
Additional Information:© 1982 The Rockefeller University Press. Received for publication 17 August 1981. Supported by grant AI 16769 from the National Institutes of Health to E. Rothenberg, and by core grant CA 14195 from the National Cancer Institute to the Armand Hammer Cancer Center. It is a pleasure to express my thanks to Joseph Trotter, for valuable advice and excellent operation of the cell sorter; and to Michael Chorney, Ulrich Hämmerling, and Fung-Win Shen for gifts of antibody. I also owe a debt of gratitude to Laura Nell, who first introduced me to the advantages of elutriation. Finally, I thank Loyd Burgess, Gunther Dennert, Robert Hyman, Bart Sefton, and Dennis Triglia for their careful criticism of the manuscript, and Ami Koide for patient and painstaking secretarial help.
Funders:
Funding AgencyGrant Number
NIHAI16769
NIHCA14195
Issue or Number:1
PubMed Central ID:PMC2186575
Record Number:CaltechAUTHORS:20170207-084442559
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170207-084442559
Official Citation:A specific biosynthetic marker for immature thymic lymphoblasts. Active synthesis of thymus-leukemia antigen restricted to proliferating cells E Rothenberg Journal of Experimental Medicine Jan 1982, 155 (1) 140-154; DOI: 10.1084/jem.155.1.140
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:74129
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:07 Feb 2017 18:57
Last Modified:08 Jul 2020 21:46

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