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Regulated expression and function of CD122 (interleukin-2/interleukin-15R-β) during lymphoid development

Reya, Tannishtha and Yang-Snyder, Julia A. and Rothenberg, Ellen V. and Carding, Simon R. (1996) Regulated expression and function of CD122 (interleukin-2/interleukin-15R-β) during lymphoid development. Blood, 87 (1). pp. 190-201. ISSN 0006-4971. http://resolver.caltech.edu/CaltechAUTHORS:20170208-110702546

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Abstract

To determine whether signaling via CD122 (interleukin-2 [IL-2]/IL-15 receptor β-chain) plays a role in regulating the expansion and differentiation of lymphocyte precursors, we have characterized its expression and evaluated its ability to influence the activity of developing lymphoid cells. A significant fraction of Sca1^+Lin^- hematopoietic stem cells in day 12 fetal liver were found to be CD122^+. CD122-mRNA^+ and IL-2-mRNA^+ cells were also localized in embryo sections within pharyngeal blood vessels adjacent to and surrounding the thymic analgen. This distribution is consistent with the migration of CD122^+ progenitor cells from the liver to the developing thymus where a majority of Sca1^+ intrathymic T-cell progenitors were CD122^+. Analysis of CD122 expression in the day 12 fetal liver revealed that the majority of B220^+ cells were CD122^+. Furthermore, CD122 expression was restricted to the earliest B220^+ cells (CD43^+CD24^-; prepro B cells; fraction A) that proliferate vigorously to IL-2 in the absence of any stromal cells, but not to IL-15. Consistent with a role for the IL-2/IL- 2R pathway in lymphocyte development is the progressive loss of B cells seen in IL-2-deficient mice. Together, these observations suggest that CD122 plays a role in regulating normal lymphocyte development in vivo.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.bloodjournal.org/content/87/1/190.longPublisherArticle
ORCID:
AuthorORCID
Rothenberg, Ellen V.0000-0002-3901-347X
Additional Information:© 1996 The American Society of Hematology. Submitted February 7, 1995; accepted August 17, 1995. Supported in part by grants from the W.W. Smith Charitable Trust, the American Cancer Society (JFRA-399), and the National Institutes of Health (AI31972). We thank Dr Randy Hardy for reviewing the manuscript, Drs M. Tanaka and M. Miyasaka for providing the TMβ1 antibody, Dr Ronald Schwartz for providing the IL-2-deficient mice, and Hank Pletcher for cell sorting. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. section 1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
W. W. Smith Charitable TrustUNSPECIFIED
American Cancer SocietyJFRA-399
NIHAI31972
Record Number:CaltechAUTHORS:20170208-110702546
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20170208-110702546
Official Citation:Regulated expression and function of CD122 (interleukin-2/interleukin- 15R-beta) during lymphoid development T Reya, JA Yang-Snyder, EV Rothenberg, SR Carding Blood Jan 1996, 87 (1) 190-201
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:74158
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:08 Feb 2017 20:03
Last Modified:08 Feb 2017 20:03

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