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Expression of Differentiation Antigens in Subpopulations of Mouse Thymocytes: Regulation at the Level of de Novo Synthesis

Rothenberg, Ellen (1980) Expression of Differentiation Antigens in Subpopulations of Mouse Thymocytes: Regulation at the Level of de Novo Synthesis. Cell, 20 (1). pp. 1-9. ISSN 0092-8674. doi:10.1016/0092-8674(80)90228-7. https://resolver.caltech.edu/CaltechAUTHORS:20170209-084208618

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Abstract

Mouse thymocytes can be divided into two broad classes by a variety of criteria, including steroid sensitivity, buoyant density, and availability of binding sites for peanut agglutinin. The major class is poorly reactive in immune functional assays, whereas the minor population does contain cells that are immunologically competent. Correlated with these functional differences, the populations differ in their displays of specific surface antigens and in their steady-state terminal deoxynucleotidyl transferase (TdT) activity. To investigate the basis for these phenotypic differences, the two populations were enriched by density gradient fractionation or by differential PNA agglutination; alternatively, the minor population was freed of the major one by in vivo cortisone treatment. These partially purified cells were then examined for their rates of de novo synthesis of four differentiation marker proteins. Incorporation of ^(35)S-methionine into H-2 (K and/or D), TL, TdT, and a Qa-region product (probably Qa-1) was monitored by immune precipitation with specific antisera followed by gel electrophoresis. The metabolic labeling interval was restricted to 30–40 min, to reflect as closely as possible instantaneous rates of synthesis. The two thymocyte populations were found to differ significantly in their production of each of these molecules. Relative to its total protein synthesis, the major population synthesized 10–20 fold less H-2, 5–10 fold more TdT, and 10–20 fold more TL than the minor population. The Qa product was synthesized in both populations, but at a slightly higher rate in the minor population. The biosynthetic rates are consistent with the reported steady-state phenotypes of the cells, and imply that changes in differentiation antigen expression during T lymphocyte development are controlled, at least in part, at a translational or pretranslational level.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0092-8674(80)90228-7DOIArticle
http://www.sciencedirect.com/science/article/pii/0092867480902287PublisherArticle
ORCID:
AuthorORCID
Rothenberg, Ellen0000-0002-3901-347X
Additional Information:© 1980 by MIT. Received 29 January 1980. I am deeply grateful to Allen Silverstone and Yvon Cayre. for exciting discussions and for the opportunity to share results prior to publication. I also thank Vair Reisner for teaching me the PNA agglutination technique; Phillips Robbins for graciously providing endo H; and Gunther Dennert, Tony Hunter, Robert Hyman, Bart Sefton, Dennis Triglia and Ian Trowbridge for their criticism of the manuscript. Finally, I am indebted to Dr; E. A. Boyse, in whose laboratory this work was initiated and without whose support it could not have been carried out. Part of this work was done while the author was a postdoctoral fellow of the Jane Coffin Childs Memorial Fund for Medical Research. It was supported by two grants from the NIH. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to Indicate this fact.
Funders:
Funding AgencyGrant Number
Jane Coffin Childs Memorial Fund for Medical ResearchUNSPECIFIED
NIHUNSPECIFIED
Issue or Number:1
DOI:10.1016/0092-8674(80)90228-7
Record Number:CaltechAUTHORS:20170209-084208618
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170209-084208618
Official Citation:Ellen Rothenberg, Expression of differentiation antigens in subpopulations of mouse thymocytes: Regulation at the level of de novo synthesis, Cell, Volume 20, Issue 1, May 1980, Pages 1-9, ISSN 0092-8674, http://dx.doi.org/10.1016/0092-8674(80)90228-7. (//www.sciencedirect.com/science/article/pii/0092867480902287)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:74190
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:09 Feb 2017 19:07
Last Modified:11 Nov 2021 05:25

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