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Lck Activity Controls CD4/CD8 T Cell Lineage Commitment

Hernández-Hoyos, Gabriela and Sohn, Sue J. and Rothenberg, Ellen V. and Alberola-Ila, José (2000) Lck Activity Controls CD4/CD8 T Cell Lineage Commitment. Immunity, 12 (3). pp. 313-322. ISSN 1074-7613. https://resolver.caltech.edu/CaltechAUTHORS:20170209-113011357

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Abstract

Thymocytes carrying MHC class I–restricted TCRs differentiate into CD8 T cells, while those recognizing MHC class II become CD4 T cells. The mechanisms underlying how MHC class recognition, coreceptor expression, and effector function are coordinated are not well understood. Since the tyrosine kinase Lck binds with more affinity to CD4 than CD8, it has been proposed as a candidate to mediate this process. By using transgenic mice with altered Lck activity, we show that thymocytes carrying a class II–restricted TCR develop into functional CD8 T cells when Lck activity is reduced. Conversely, thymocytes carrying a class I–restricted TCR develop into functional CD4 T cells when Lck activity is increased. These results directly show that quantitative differences in the Lck signal control the CD4/CD8 lineage decision.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S1074-7613(00)80184-3DOIArticle
http://www.sciencedirect.com/science/article/pii/S1074761300801843PublisherArticle
ORCID:
AuthorORCID
Rothenberg, Ellen V.0000-0002-3901-347X
Additional Information:© 2000 Cell Press. Received 16 November 1999, Revised 17 January 2000. We thank R. M. Perlmutter for providing the dLGF and dLGKR mice; R. Diamond for the cell sorts; H. Wang for the PCR primers; D. Miller, L. Anonuevo, and B. Kennedy for animal husbandry; and S. Kovats, P. W. Sternberg, P. J. Bjorkman, and A. Y. Rudensky for suggestions and helpful discussions. G. H-H. was supported by funding from the Stowers Institute for Medical Research. J. A-I. is a Pew Scholar. This work was supported by the Pew Scholar Program in the biomedical sciences and by National Institutes of Health grant number AI45072-01A1 to J. A-I.
Funders:
Funding AgencyGrant Number
Stowers Institute for Medical ResearchUNSPECIFIED
Pew Charitable TrustUNSPECIFIED
NIHAI45072-01A1
Issue or Number:3
Record Number:CaltechAUTHORS:20170209-113011357
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170209-113011357
Official Citation:Gabriela Hernández-Hoyos, Sue J Sohn, Ellen V Rothenberg, José Alberola-Ila, Lck Activity Controls CD4/CD8 T Cell Lineage Commitment, Immunity, Volume 12, Issue 3, 1 March 2000, Pages 313-322, ISSN 1074-7613, http://dx.doi.org/10.1016/S1074-7613(00)80184-3. (//www.sciencedirect.com/science/article/pii/S1074761300801843)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:74194
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:09 Feb 2017 21:48
Last Modified:03 Oct 2019 16:35

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