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IL-2 receptor inducibility is blocked in cortical-type thymocytes

Boyer, Paul D. and Rothenberg, Ellen V. (1988) IL-2 receptor inducibility is blocked in cortical-type thymocytes. Journal of Immunology, 140 (9). pp. 2886-2892. ISSN 0022-1767. http://resolver.caltech.edu/CaltechAUTHORS:20170210-113249636

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Abstract

Activation of mature T cells induces the expression of high affinity receptors for the T cell growth hormone, IL-2. A short term assay was used to determine the capacity of murine thymocyte subpopulations to express the gene encoding the 55-kDa chain of the IL-2R after stimulation in vitro. Thymocytes were cultured in the presence or absence of phorbol ester and calcium ionophore for 20 h, stained with antibodies against the IL-2R or the MHC Ag, H-2K, and analyzed by FACS. At least 90% of CD4-CD8- thymocytes were competent to express IL-2R, implying that this functional response is acquired early in development. However, at least 80% of cortical thymocytes were unable to express IL-2R before or after stimulation. Additional fractionation of the nonresponsive cells indicated that they constitute the great majority of the CD4+CD8+ population, including virtually all of the CD4+CD8+ proliferating blasts. Most nonresponsive cells remain viable and react to the chemical stimuli by increasing their surface expression of H-2K. The inability of most cortical thymocytes to express IL-2R suggests that a discrete loss of function occurs in most cells as they differentiate from CD4-CD8- precursors into the CD4+CD8+ class. The inferred loss of function is mediated at the level of IL-2 RNA accumulation and may be correlated with a pleiotropic alteration in physiologic response pathways.


Item Type:Article
Related URLs:
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http://www.jimmunol.org/content/140/9/2886PublisherArticle
ORCID:
AuthorORCID
Rothenberg, Ellen V.0000-0002-3901-347X
Additional Information:© 1988 American Association of Immunologists. Received for publication October 1, 1988. Accepted for publication January 25, 1988. This work was supported in part by a California University-wide AIDS Tasks Force grant to E.V.R. (AIDS-11) from the U.S. Public Health Service. The flow cytometry facility was supported by Grant CA32911 from the National Cancer Institute and by an Instrumentation Support Grant from the Markey Developmental Biology Program at Caltech. We gratefully acknowledge the expert flow cytometric analysis done by Rochelle Diamond and Patrick Koen. We are also extremely grateful to Victoria Wong at the University of Southern California for FACS time when our machine was down. We thank Drs. D. Anderson, E. Davidson, L. Hood, and K. McGuire for valuable critical comments on the manuscript. D r. James Lugo conducted the initial IL-2R analysis of double-negative thymocytes. We thank the members of this laboratory for helpful suggestions.
Funders:
Funding AgencyGrant Number
California University-wide AIDS Tasks ForceAIDS-11
National Cancer InstituteUNSPECIFIED
NIHCA32911
Lucille P. Markey Charitable TrustUNSPECIFIED
Record Number:CaltechAUTHORS:20170210-113249636
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20170210-113249636
Official Citation:IL-2 receptor inducibility is blocked in cortical-type thymocytes. P D Boyer, E V Rothenberg The Journal of Immunology May 1, 1988, 140 (9) 2886-2892
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:74208
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:10 Feb 2017 21:47
Last Modified:10 Feb 2017 21:47

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