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Lyt-2 glycoprotein is synthesized as a single molecular species

Rothenberg, E. and Triglia, D. (1983) Lyt-2 glycoprotein is synthesized as a single molecular species. Journal of Experimental Medicine, 157 (1). pp. 365-370. ISSN 0022-1007. PMCID PMC2186898. https://resolver.caltech.edu/CaltechAUTHORS:20170210-131601098

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Abstract

We investigated the possibility that the Lyt-2 molecules made by uncloned mouse T lymphocytes would show variable primary structures like those of immunoglobulins. Newly synthesized Lyt-2/3 complexes were found to include only two major components, both discrete glycoproteins with apparent molecular weights of 31,000 (31 K) and 35,000 (35 K). When products of Lyt-2.1 and Lyt-2.2 thymocytes were compared by two-dimensional nonequilibrium pH gradient electrophoresis and sodium dodecyl sulfate polyacrylamide gel electrophoresis, the isoelectric points of the 35 K molecules were different; thus, the 35 K component was likely to be encoded by the Lyt-2 locus itself. However, the 35 K molecules made by any one genotype were homogeneous in charge as well as in size. The homogeneity was obscured rapidly by post-translational modification. Most strikingly, within 30 min of initial synthesis, these processing events generated the conspicuous array of microheterogeneous products that form the "38 K" component of cell-surface Lyt-2/3.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1084/jem.157.1.365DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2186898/PubMed CentralArticle
ORCID:
AuthorORCID
Rothenberg, E.0000-0002-3901-347X
Additional Information:© 1983 Rockefeller University Press. After the Initial Publication Period, RUP will grant to the public the non-exclusive right to copy, distribute, or display the Article under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode, or updates thereof. Supported by grants CA 31667, AI 16769, AI 19752, and CA 14195 from the National Institutes of Health. We would like to thank Dr. Susan Swain and Dr. Richard Dutton for congenic mice and Dr. Leonard Herzenberg and Dr. Ulrich Hammerling for monoclonal antibodies. Endo H was the generous gift of Dr. Phillips Robbins.
Funders:
Funding AgencyGrant Number
NIHCA31667
NIHAI16769
NIHAI19752
NIHCA14195
Issue or Number:1
PubMed Central ID:PMC2186898
Record Number:CaltechAUTHORS:20170210-131601098
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170210-131601098
Official Citation:Lyt-2 glycoprotein is synthesized as a single molecular species. E Rothenberg, D Triglia Journal of Experimental Medicine Jan 1983, 157 (1) 365-370; DOI: 10.1084/jem.157.1.365
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:74212
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:11 Feb 2017 01:25
Last Modified:08 Jul 2020 21:46

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