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Synthesis and processing of molecules bearing thymus leukemia antigen

Rothenberg, E. and Boyser, Edward A. (1979) Synthesis and processing of molecules bearing thymus leukemia antigen. Journal of Experimental Medicine, 150 (4). pp. 777-791. ISSN 0022-1007. http://resolver.caltech.edu/CaltechAUTHORS:20170213-072709317

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Abstract

Thymus-leukemia (TL) antigens are expressed in murine lymphocytes under strict developmental regulation. To elucidate the molecular basis of TL expression, we have identified the molecular species that react with TL antiserum. At least three species can be resolved by metabolic radiolabeling of thymocytes and ASL1 leukemia cells, lysis, immune precipitation, and sodium dodecyl sulfate-polyacrylamide. After a brief incubation with [35S]methionine, the only radioactive molecule recognized by TL antiserum is a homogeneous species with an apparent Mr of 45,000 daltons. This molecule, 45K TL, includes high-mannose-type carbohydrate attached to a 45,000 dalton glycosidase-resistant backbone. In this form, 45K, it is never exposed on the cell surface. If pulse-labeled cells are further incubated with nonradioactive methionine before lysis, however, radioactivity disappears from the 45K TL species and appears in the slower migrating species 46K and 48K TL. Thus, 46K and 48K appear to represent products generated from the 45K TL precursor by posttranslational modification. These TL forms are displayed on the cell surface; they lack high-mannose carbohydrate but evidently include acidic complex-type carbohydrate. Normal thymocytes from Qa:Tla-negative mice lack not only the surface forms of TL but also the intracellular 45K TL form. Peripheral lymphoid cells of Qa:Tla-positive mice synthesize none of these TL species. But the TL antiserum, which contains Qa antibody, recognizes a distinct gene product in spleen and thymus of Qa-Tla-positive mice. In its pulse-labeled form, this molecule, which may represent Qa-1, has an apparent Mr of 44,000 daltons, and consists of a glycosidase-resistant polypeptide core of only 35,000 daltons linked to more high mannose carbohydrate than 45K TL.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1084/jem.150.4.777DOIArticle
http://jem.rupress.org/content/150/4/777.longPublisherArticle
ORCID:
AuthorORCID
Rothenberg, E.0000-0002-3901-347X
Additional Information:© 1979 Rockefeller University Press. After the Initial Publication Period, RUP will grant to the public the non-exclusive right to copy, distribute, or display the Article under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode, or updates thereof. Received for publication 6 April 1979. Supported in part by grant CA 25738 from the National Cancer Institute. Recipient of fellowship from the Jane Coffin Childs Memorial Fund for Medical Research. American Cancer Society Research Professor of Cell Surface Immunogenetics. We are indebted to O. N. Witte, L. M. Silver, and D. J. Donoghue for technical advice and helpful discussions throughout this work, and to R. Hyman, P. W. Robbins, and L. E. Hood for cells and valuable reagents. We also thank D. Bennett for her generous interest in this work.
Funders:
Funding AgencyGrant Number
NIHCA25738
Jane Coffin Childs Memorial Fund for Medical ResearchUNSPECIFIED
American Cancer SocietyUNSPECIFIED
Record Number:CaltechAUTHORS:20170213-072709317
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20170213-072709317
Official Citation:Synthesis and processing of molecules bearing thymus leukemia antigen. E Rothenberg, E A Boyse Journal of Experimental Medicine Oct 1979, 150 (4) 777-791; DOI: 10.1084/jem.150.4.777
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:74222
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:13 Feb 2017 15:59
Last Modified:13 Feb 2017 15:59

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