Lindenboim, Liora and Diamond, Rochelle and Rothenberg, Ellen and Stein, Reuven (1995) Apoptosis Induced by Serum Deprivation of PC12 Cells Is Not Preceded by Growth Arrest and Can Occur at Each Phase of the Cell Cycle. Cancer Research, 55 (6). pp. 1242-1247. ISSN 0008-5472. https://resolver.caltech.edu/CaltechAUTHORS:20170213-081245647
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Abstract
Previous studies have shown that PC12 cells undergo apoptosis (programmed cell death) when deprived of serum. In the present study, we examined the relationship of this death process to the cell cycle. PC12 cell populations synchronized at different, specific phases of the cell cycle exhibit similar kinetics of cell death following deprivation of serum. Flow cytometry analysis was used to examine the levels of apoptotic death in these cell populations in relationship to their progression in the cell cycle during the course of serum deprivation. Such analysis revealed that the cells the during the G_o-G_1, S, and perhaps G_2-M phases and at the G_2 to G_1 transition. These results, therefore, suggest that the death of synchronized, serum-deprived PC12 cells occurs throughout the cell cycle and is not dependent on growth arrest. Flow cytometry methodology (acridine orange staining), which determines the RNA content of cells in relationship to their position in the cell cycle, was used to address these questions in nonsynchronized cells. These experiments revealed that, upon serum deprivation, an immediate loss of RNA occurred from cells in G_1, S, and G_2-M phases. This loss is accompanied by a slower appearance of cells with degraded DNA content. These results show that cells from all phases of the cell cycle are damaged upon serum deprivation and thus suggest that the apoptotic cell death of nonsynchronized PC12 cells may occur from each phase of the cell cycle.
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Additional Information: | © 1995 American Association for Cancer Research. Received 8/12/94; accepted 1/18/95. This work was supported by Grant 90-00347 from the United States-Israel Binational Science Foundation (Jerusalem, Israel). | ||||||
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Issue or Number: | 6 | ||||||
Record Number: | CaltechAUTHORS:20170213-081245647 | ||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20170213-081245647 | ||||||
Official Citation: | Apoptosis Induced by Serum Deprivation of PC12 Cells Is Not Preceded by Growth Arrest and Can Occur at Each Phase of the Cell Cycle Liora Lindenboim, Rochelle Diamond, Ellen Rothenberg and Reuven Stein Cancer Res March 15 1995 (55) (6) 1242-1247 | ||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||
ID Code: | 74225 | ||||||
Collection: | CaltechAUTHORS | ||||||
Deposited By: | Ruth Sustaita | ||||||
Deposited On: | 13 Feb 2017 16:47 | ||||||
Last Modified: | 03 Oct 2019 16:36 |
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