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Genome-linked protein VPg of poliovirus is present as free VPg and VPg-pUpU in poliovirus-infected cells

Crawford, Nigel M. and Baltimore, David (1983) Genome-linked protein VPg of poliovirus is present as free VPg and VPg-pUpU in poliovirus-infected cells. Proceedings of the National Academy of Sciences of the United States of America, 80 (24). pp. 7452-7455. ISSN 0027-8424. PMCID PMC389969. doi:10.1073/pnas.80.24.7452.

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VPg is a virus-encoded protein covalently attached to the 5' end of poliovirus virion RNA. We have used antibody prepared against chemically synthesized VPg to detect two forms of VPg in infected cells. Both forms were specifically immunoprecipitated from lysates of infected cells labeled with [3H]leucine. One appears to be unmodified VPg because it had the same electrophoretic mobility as synthetic VPg. The other had a larger apparent molecular weight than VPg and could be labeled in vivo with 32Pi. Its structure is VPg-pUpU, the UMP dinucleotide being attached to VPg via a phosphodiester bond to tyrosine, the third amino acid from the NH2 terminus of VPg. This structure is identical to that found at the 5' end of virion and minus-strand RNA.

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Baltimore, David0000-0001-8723-8190
Additional Information:© 1983 by the National Academy of Sciences. Contributed by David Baltimore, September 15, 1983. We thank Marie Chow, Peter Sarnow, Karla Kirkegaard, Gordon Foulkes, and Vincent Racaniello for their help and suggestions. N.M.C. was a predoctoral fellow of the National Science Foundation. D.B. is an American Cancer Society Research Professor. This investigation was supported by grants from the National Institutes of Health. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funding AgencyGrant Number
NSF Graduate Research FellowshipUNSPECIFIED
American Cancer SocietyUNSPECIFIED
Subject Keywords:RNA replication; picornavirus
Issue or Number:24
PubMed Central ID:PMC389969
Record Number:CaltechAUTHORS:CRApnas83
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7423
Deposited By: Tony Diaz
Deposited On:13 Feb 2007
Last Modified:08 Nov 2021 20:42

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