CaltechAUTHORS
  A Caltech Library Service

Changes in inducibility of IL-2 receptor alpha-chain and T cell-receptor expression during thymocyte differentiation in the mouse

Boyer, Paul D. and Diamond, Rochelle A. and Rothenberg, Ellen V. (1989) Changes in inducibility of IL-2 receptor alpha-chain and T cell-receptor expression during thymocyte differentiation in the mouse. Journal of Immunology, 142 (12). pp. 4124-4130. ISSN 0022-1767. http://resolver.caltech.edu/CaltechAUTHORS:20170214-152458305

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:20170214-152458305

Abstract

Within the thymus, developing T cells must acquire the competence to respond to appropriate signals by inducing the expression of genes required for immunologic function; one such gene encodes the 55-kDa-chain of the IL-2R (IL-2R alpha). Previously, we showed that most cortical-type thymocytes lack the competence to make this particular response, while most medullary-type cells respond like mature T lymphocytes. The noninducibility of cortical-type cells was striking, because most of their presumed precursors were inducible. To test the relationship between this apparent loss of competence and the positive and negative selection processes that may occur in the thymic cortex, we have assayed the inducibility of thymocyte populations, staged carefully with respect to their expression of TCR. Using size fractionation to enrich for dividing cells, we concentrated and thereby revealed defined developmental intermediates. We report that, although CD4+CD8- thymocytes behave as mature T cells, a significant fraction of CD4-CD8+ cells are noninducible. These noninducible thymocytes are dividing cells, which appear to be in a major developmental continuum between CD4-CD8- blasts and CD4+CD8+ blasts. Furthermore, the noninducible blasts as yet lack surface TCR expression. We also demonstrate the functional similarity of these CD4-CD8+ cells to a major subset of dividing CD4-CD8- precursor cells, which appear to have lost IL-2R alpha expression. These results suggest that precursors of cortical thymocytes lose competence to be induced to express IL-2R alpha several stages before their acquisition of cell-surface TCR complexes. The implications of this characterization are discussed in terms of the possible relationships between IL-2R alpha gene regulation and intrathymic fate determination.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.jimmunol.org/content/142/12/4121PublisherArticle
ORCID:
AuthorORCID
Rothenberg, Ellen V.0000-0002-3901-347X
Additional Information:© 1989 The American Association of Immunologists. Received for publication January 23. 1989. Accepted for publication March 24. 1989. This work was supported in part by State of California University wide AIDS Tasks Force grants to E. V. R. (AIDS-11 and R86CT001) and in part by grant A119752 from the U.S. Public Health Service. The flow cytometry facility was suported by Grant CA 32911 from the National Cancer Institute and by an Instrumentation Support Grant from Markey Developmental Biology Program at Caltech.
Funders:
Funding AgencyGrant Number
California University-wide AIDS Tasks ForceAIDS-11
California University-wide AIDS Tasks ForceR86CT001
NIHAI19752
NIHCA32911
Lucille P. Markey Charitable TrustUNSPECIFIED
Record Number:CaltechAUTHORS:20170214-152458305
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20170214-152458305
Official Citation:Changes in inducibility of IL-2 receptor alpha-chain and T cell-receptor expression during thymocyte differentiation in the mouse. P D Boyer, R A Diamond, E V Rothenberg The Journal of Immunology June 15, 1989, 142 (12) 4121-4130
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:74307
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:15 Feb 2017 15:47
Last Modified:15 Feb 2017 15:47

Repository Staff Only: item control page