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Inducibility of interleukin-2 RNA expression in individual mature and immature T lymphocytes

McGuire, Kathleen L. and Rothenberg, Ellen V. (1987) Inducibility of interleukin-2 RNA expression in individual mature and immature T lymphocytes. EMBO Journal, 6 (4). pp. 939-946. ISSN 0261-4189. PMCID PMC553486.

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Expression of the gene for the T-cell growth hormone, interleukin 2 (IL2), is subject to at least two types of control. It is not only tissue specific, i.e. restricted to T lymphocytes, but also strictly dependent upon activation of the producing T cell. In mature cells, IL2 production is usually triggered via the cell surface receptor for antigen. To study the regulation of the murine IL2 gene in T-cell populations of differing stages of maturation, we have used a calcium ionophore in conjunction with the phorbol ester, TPA, to stimulate IL2 gene transcription while bypassing the requirement for triggering through a mature cell surface receptor. We have combined in situ hybridization with RNA probe protection analyses to quantitate accumulated cytoplasmic IL2 RNA and to identify the cells capable of inducing the IL2 gene in mature, immature and precursor T-cell populations. We report evidence for a distinction between the IL2 mRNA induction responses of different T cells, according to their maturation state and/or functional subclass. Mature splenic T cells that make IL2 can accumulate IL2 transcripts to more than 100 copies per cell. However, we find that many T-lineage cells, especially in immature populations, show induction-dependent IL2 gene expression but only accumulate low levels of IL2 mRNA per cell.

Item Type:Article
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URLURL TypeDescription CentralArticle
Rothenberg, Ellen V.0000-0002-3901-347X
Additional Information:© 1987 EMBO. Received on November 20, 1986; revised on January 28, 1987. The authors would like to thank Rochelle Diamond and Sharon Kochik for excellent technical assistance, Drs Norman Davidson, Elliott Meyerowitz, Lee Hood and Eric Davidson for critical reviews and valuable comments on the manuscript, and Tom Novak and Drs Barry Caplan, Christine Kinnon and James Lugo for helpful discussions. We would like also to thank Dr Andy Cameron for help with photomicroscopy. KLM is supported by NCI fellowship No. CA07876 and this work was supported by grants No. AI-19752 and CA-39605 from the National Institutes of Health. The flow cytometry facility was supported by grants CA 32911 from NCI and by an Instrumentation Support Grant from the Markey Foundation.
Funding AgencyGrant Number
NIH Postdoctoral FellowshipCA07876
Lucille P. Markey Charitable TrustUNSPECIFIED
Subject Keywords:interleukin-2 / T lymphocytes/ thymus / in situ hybridization / developmental gene regulation
Issue or Number:4
PubMed Central ID:PMC553486
Record Number:CaltechAUTHORS:20170216-143807033
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Official Citation:McGuire KL, Rothenberg EV. Inducibility of interleukin-2 RNA expression in individual mature and immature T lymphocytes. The EMBO Journal. 1987;6(4):939-946.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:74389
Deposited By: George Porter
Deposited On:17 Feb 2017 00:17
Last Modified:03 Oct 2019 16:38

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