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Sequence-specific recognition of double helical nucleic acids by proteins

Seeman, Nadrian C. and Rosenberg, John M. and Rich, Alexander (1976) Sequence-specific recognition of double helical nucleic acids by proteins. Proceedings of the National Academy of Sciences of the United States of America, 73 (3). pp. 804-808. ISSN 0027-8424.

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The base pairs in double helical nucleic acids have been compared to see how they can be recognized by proteins. We conclude that a single hydrogen bond is inadequate for uniquely identifying any particular base pair, as this leads to numerous degeneracies. However, using two hydrogen bonds, fidelity of base pair recognition may be achieved. We propose specific amino-acid side chain interactions involving two hydrogen bonds as a component of the recognition system for base pairs. In the major groove we suggest that asparagine or glutamine binds to adenine of the base pair, or arginine binds to guanine. In the minor groove, we suggest an interaction between asparagine or glutamine with guanine of the base pair. We also discuss the role that ions and other amino-acid side chains may play in recognition interactions.

Item Type:Article
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Seeman, Nadrian C.0000-0002-9680-4649
Additional Information:© 1976 by the National Academy of Sciences. Contributed by Alexander Rich, December 10, 1975. This research was supported by research grants from the National Institutes of Health, the National Science Foundation, the National Aeronautics and Space Administration, and the American Cancer Society. N.C.S. is a fellow of the National Institutes of Health.
Subject Keywords:base pairs; hydrogen bonding; recognition fidelity; ion binding
Issue or Number:3
Record Number:CaltechAUTHORS:SEEpnas76
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7442
Deposited By: Tony Diaz
Deposited On:21 Feb 2007
Last Modified:09 Mar 2020 13:19

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