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Nonlinear ultrasound imaging of nanoscale acoustic biomolecules

Maresca, David and Lakshmanan, Anupama and Lee-Gosselin, Audrey and Melis, Johan M. and Ni, Yu-Li and Bourdeau, Raymond W. and Kochmann, Dennis M. and Shapiro, Mikhail G. (2017) Nonlinear ultrasound imaging of nanoscale acoustic biomolecules. Applied Physics Letters, 110 (7). Art. No. 073704. ISSN 0003-6951. PMCID PMC5315666. https://resolver.caltech.edu/CaltechAUTHORS:20170221-114609641

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Abstract

Ultrasound imaging is widely used to probe the mechanical structure of tissues and visualize blood flow. However, the ability of ultrasound to observe specific molecular and cellular signals is limited. Recently, a unique class of gas-filled protein nanostructures called gas vesicles (GVs) was introduced as nanoscale (∼250 nm) contrast agents for ultrasound, accompanied by the possibilities of genetic engineering, imaging of targets outside the vasculature and monitoring of cellular signals such as gene expression. These possibilities would be aided by methods to discriminate GV-generated ultrasound signals from anatomical background. Here, we show that the nonlinear response of engineered GVs to acoustic pressure enables selective imaging of these nanostructures using a tailored amplitude modulation strategy. Finite element modeling predicted a strongly nonlinear mechanical deformation and acoustic response to ultrasound in engineered GVs. This response was confirmed with ultrasound measurements in the range of 10 to 25 MHz. An amplitude modulation pulse sequence based on this nonlinear response allows engineered GVs to be distinguished from linear scatterers and other GV types with a contrast ratio greater than 11.5 dB. We demonstrate the effectiveness of this nonlinear imaging strategy in vitro, in cellulo, and in vivo.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://doi.org/10.1063/1.4976105DOIArticle
http://aip.scitation.org/doi/abs/10.1063/1.4976105PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5315666/PubMed CentralArticle
ORCID:
AuthorORCID
Maresca, David0000-0002-4921-6406
Lakshmanan, Anupama0000-0002-6702-837X
Lee-Gosselin, Audrey0000-0002-2431-2741
Bourdeau, Raymond W.0000-0003-2202-1980
Kochmann, Dennis M.0000-0002-9112-6615
Shapiro, Mikhail G.0000-0002-0291-4215
Additional Information:© 2017 American Institute of Physics. Received 31 October 2016; accepted 29 January 2017; published online 17 February 2017. The authors thank Dan Piraner for assistance with the GV phantom mold design, and Stuart Foster and Emmanuel Chérin for helpful discussions. This research was supported by the National Institutes of Health (R01-EB018975). D.M. is supported by the Human Frontiers Science Program Cross-Disciplinary Postdoctoral Fellowship (Award No. LT000637/2016). A.L. is supported by the NSF graduate research fellowship (Award No. 1144469). Y-L.N. is supported by the Taipei Veterans General Hospital / National Yang-Ming University Excellent Physician Scientists Cultivation Program, No. 103-Y-A-003. Research in the Shapiro laboratory is also supported by the Heritage Medical Research Institute, Burroughs Wellcome Career Award at the Scientific Interface, the Pew Scholarship in the Biomedical Sciences, and the Packard Fellowship for Science and Engineering.
Group:Heritage Medical Research Institute
Funders:
Funding AgencyGrant Number
NIHR01-EB018975
Human Frontier Science ProgramLT000637/2016
NSF Graduate Research FellowshipDGE-1144469
Taipei Veterans General Hospital / National Yang-Ming University103-Y-A-003
Heritage Medical Research InstituteUNSPECIFIED
Burroughs Wellcome FundUNSPECIFIED
Pew Charitable TrustUNSPECIFIED
David and Lucile Packard FoundationUNSPECIFIED
Issue or Number:7
PubMed Central ID:PMC5315666
Record Number:CaltechAUTHORS:20170221-114609641
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170221-114609641
Official Citation:Nonlinear ultrasound imaging of nanoscale acoustic biomolecules David Maresca, Anupama Lakshmanan, Audrey Lee-Gosselin, Johan M. Melis, Yu-Li Ni, et al. Appl. Phys. Lett. 110, 073704 (2017); http://doi.org/10.1063/1.4976105
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:74424
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:21 Feb 2017 20:28
Last Modified:22 Nov 2019 22:46

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