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Single-cell transcriptomics reveals receptor transformations during olfactory neurogenesis

Hanchate, Naresh K. and Kondoh, Kunio and Lu, Zhonglua and Kuang, Donghui and Ye, Xiaolan and Qiu, Xiaojie and Pachter, Lior and Trapnell, Cole and Buck, Linda B. (2015) Single-cell transcriptomics reveals receptor transformations during olfactory neurogenesis. Science, 350 (6265). pp. 1251-1255. ISSN 0036-8075. PMCID PMC5642900. doi:10.1126/science.aad2456.

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The sense of smell allows chemicals to be perceived as diverse scents. We used single neuron RNA-Sequencing (RNA-Seq) to explore developmental mechanisms that shape this ability as nasal olfactory neurons mature in mice. Most mature neurons expressed only one of the roughly 1000 odorant receptor genes (Olfrs) available, and that at high levels. However, many immature neurons expressed low levels of multiple Olfrs. Coexpressed Olfrs localized to overlapping zones of the nasal epithelium, suggesting regional biases, but not to single genomic loci. A single immature neuron could express Olfrs from up to seven different chromosomes. The mature state in which expression of Olfr genes is restricted to one per neuron emerges over a developmental progression that appears independent of neuronal activity requiring sensory transduction molecules.

Item Type:Article
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URLURL TypeDescription Materials Expression Omnibus CentralArticle
Pachter, Lior0000-0002-9164-6231
Additional Information:© 2015 American Association for the Advancement of Science. 13 August 2015; accepted 27 October 2015. We thank J. Delrow, A. Marty, and A. Dawson at the Fred Hutchinson Cancer Research Center (FHCRC) Genomics Facility for assistance with RNA-seq; M. Fitzgibbon and J. Davidson at the FHCRC Bioinformatics Resource for early assistance with sequence analyses; and J. Vasquez and the FHCRC Scientific Imaging Facility for help with confocal microscopy. We also thank members of the Buck laboratory for helpful discussions. This work was supported by the Howard Hughes Medical Institute (L.B.B.), NIH grants R01 DC009324 (L.B.B.) and DP2 HD088158 (C.T.), an Alfred P. Sloan Fellowship (C.T.), and a Dale F. Frey Award for Breakthrough Scientists from the Damon Runyon Cancer Research Foundation (C.T.). L.B.B. is on the Board of Directors of International Flavors & Fragrances. The supplementary materials contain additional data. N.K.H., C.T., and L.B.B. designed the research; N.K.H. and C.T. performed the research; N.K.H., C.T., K.K., Z.L., D.K., X.Y., X.Q., and L.B.B. analyzed the data; L.P. provided guidance; and N.K.H, C.T., and L.B.B. wrote the paper. Raw sequencing data related to this study have been archived in the Gene Expression Omnibus (GEO) database under accession number GSE75413 (available at
Funding AgencyGrant Number
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
NIHR01 DC009324
NIHDP2 HD088158
Alfred P. Sloan FoundationUNSPECIFIED
Damon Runyon Cancer Research FoundationUNSPECIFIED
Issue or Number:6265
PubMed Central ID:PMC5642900
Record Number:CaltechAUTHORS:20170303-130217211
Persistent URL:
Official Citation:Single-cell transcriptomics reveals receptor transformations during olfactory neurogenesis BY NARESH K. HANCHATE, KUNIO KONDOH, ZHONGHUA LU, DONGHUI KUANG, XIAOLAN YE, XIAOJIE QIU, LIOR PACHTER, COLE TRAPNELL, LINDA B. BUCK Science 04 Dec 2015: Vol. 350, Issue 6265, pp. 1251-1255 DOI: 10.1126/science.aad2456
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:74703
Deposited By: George Porter
Deposited On:03 Mar 2017 21:48
Last Modified:11 Nov 2021 05:29

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