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The cascading, interrelated roles of interleukin-1, interleukin-2, and interleukin-6 in murine anti-CD3-driven T cell proliferation

Pankewycz, Oleh G. and Yui, Mary and Kelley, Vicki E. and Strom, Terry B. (1990) The cascading, interrelated roles of interleukin-1, interleukin-2, and interleukin-6 in murine anti-CD3-driven T cell proliferation. Clinical Immunology and Immunopathology, 55 (1). pp. 67-85. ISSN 0090-1229. https://resolver.caltech.edu/CaltechAUTHORS:20170303-144045044

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Abstract

T cell stimulation occurs following interaction of T cell receptor (TcR) with processed antigen presented by autologous accessory cells (AC). The effects of antigen stimulation on T cells are mimicked by monoclonal antibodies (Mabs) defining proteins of the TcR-CD3 complex. In this study, we examine the roles of T cell density, AC density, divalent and polyvalent forms of anti-CD3 Mab, and the cytokines interleukin (IL)-1, IL-2, and IL-6 in T cell activation and proliferation. Stringently AC-depleted T cells do not proliferate in response to con A or divalent anti-CD3; however, polyvalent anti-CD3 provides a powerful signal for isolated resting T cell proliferation. Recombinant (r)IL-2 strongly amplifies T cell proliferation in response to anti-CD3, whereas rIL-1 exerts no direct effects on anti-CD3-stimulated T cells. In the presence of AC, however, rIL-1 augments T cell proliferation to anti-CD3. Recombinant IL-6 promotes T cell proliferation among T cells stimulated with polyvalent but not divalent anti-CD3. As deduced by Northern blot analysis, rIL-1 increases cytoplasmic levels of IL-6 mRNA in AC. Recombinant IL-6, in turn, amplifies the accumulation of stable IL-2 transcripts in purified T cells stimulated with polyvalent anti-CD3. Hence, IL-1, IL-6, and IL-2 support T cell proliferation through cascading effects at the level of gene transcription. The cytokines evaluated in this study, however, do not fully reconstitute AC functions in promoting anti-CD3 Mab T cell proliferation.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0090-1229(90)90069-3DOIArticle
http://www.sciencedirect.com/science/article/pii/0090122990900693PublisherArticle
ORCID:
AuthorORCID
Yui, Mary0000-0002-3136-2181
Additional Information:© 1990 Elsevier Inc. Received 8 May 1989, Accepted 7 November 1989. This work was supported by NIH Grants AM 35149, DK 33929, and AI 122882.
Funders:
Funding AgencyGrant Number
NIHAM 35149
NIHDK 33929
NIHAI 122882
Issue or Number:1
Record Number:CaltechAUTHORS:20170303-144045044
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170303-144045044
Official Citation:Oleh G. Pankewycz, Mary Yui, Vicki E. Kelley, Terry B. Strom, The cascading, interrelated roles of interleukin-1, interleukin-2, and interleukin-6 in murine anti-CD3-driven T cell proliferation, Clinical Immunology and Immunopathology, Volume 55, Issue 1, 1990, Pages 67-85, ISSN 0090-1229, http://dx.doi.org/10.1016/0090-1229(90)90069-3. (http://www.sciencedirect.com/science/article/pii/0090122990900693)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:74723
Collection:CaltechAUTHORS
Deposited By: Donna Wrublewski
Deposited On:24 Mar 2017 18:47
Last Modified:03 Oct 2019 16:42

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