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Increased tumor necrosis factor and IL-1 beta gene expression in the kidneys of mice with lupus nephritis

Boswell, Jaquie M. and Yui, Mary A. and Burt, David W. and Kelley, Vicki E. (1988) Increased tumor necrosis factor and IL-1 beta gene expression in the kidneys of mice with lupus nephritis. Journal of Immunology, 141 (9). pp. 3050-3054. ISSN 0022-1767.

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Because TNF and IL-1 can initiate immunologic and inflammatory events alone or synergistically, a local increase in the levels of one or both of these cytokines in vivo may cause irreparable tissue damage. The purpose of this study was to evaluate local TNF and IL-1 beta gene expression in vivo in the kidneys of MRL-Ipr mice with autoimmune lupus nephritis. TNF mRNA was detected in the renal cortex of MRL-Ipr mice but was not present in the cortex of normal congenic MRL-++ or C3H/FeJ mice. MRL-Ipr mice with lupus nephritis expressed higher amounts of TNF mRNA compared with MRL-Ipr mice prior to disease. In addition, freshly isolated, unstimulated glomeruli from MRL-Ipr mice with nephritis were found to secrete detectable levels of TNF, whereas glomeruli from MRL-++ mice did not. IL-1 beta mRNA, present in the renal cortex of C3H/FeJ, MRL-++, and young MRL-Ipr mice with normal kidneys, was also more abundantly expressed in MRL-Ipr mice with nephritis. Cultured macrophages from glomeruli of mice with nephritis were found to express TNF and IL-1 beta mRNA and product. These macrophages are prominent only in MRL-Ipr mice with renal disease and are the likely source of increased gene expression for both cytokines.

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Yui, Mary A.0000-0002-3136-2181
Additional Information:© 1988 American Association of Immunologists. Received for publication January 19, 1988. Accepted for publication July 26, 1988. This work was supported by Grant DK-36149 from the National Institutes of Health. and the Pennsylvania and Massachusetts Lupus Foundations. We thank Drs. Charles B. Carpenter, Stefan Endres, and Rudolf Wuthrich for helpful comments on the manuscript. We also wish to thank Chu Chang for excellent technical assistance, and Corinne Kennedy for careful secretarial assistance. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
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Pennsylvania Lupus FoundationUNSPECIFIED
Massachusetts Lupus FoundationUNSPECIFIED
Issue or Number:9
Record Number:CaltechAUTHORS:20170303-160017956
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Official Citation:Increased tumor necrosis factor and IL-1 beta gene expression in the kidneys of mice with lupus nephritis. J M Boswell, M A Yui, D W Burt, V E Kelley The Journal of Immunology November 1, 1988, 141 (9) 3050-3054
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:74741
Deposited By: Donna Wrublewski
Deposited On:18 Mar 2017 03:55
Last Modified:03 Oct 2019 16:42

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