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Schistosoma mansoni: Agglutination of sporocysts, and formation of gels on miracidia transforming in plasma of Biomphalaria glabrata

Loker, Eric S. and Yui, Mary A. and Bayne, Christopher J. (1984) Schistosoma mansoni: Agglutination of sporocysts, and formation of gels on miracidia transforming in plasma of Biomphalaria glabrata. Experimental Parasitology, 58 (1). pp. 56-62. ISSN 0014-4894. https://resolver.caltech.edu/CaltechAUTHORS:20170303-165004725

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Abstract

Cellular interactions, leading to cell-mediated cytotoxicity when Biomphalaria glabrata hemocytes encapsulate Schistosoma mansoni sporocysts, have been investigated. Rabbit antibodies (IgG), when bound to antigens on sporocyst surfaces, prevent the normal cytoadherence (CA) of hemocytes from both susceptible and resistant host snails. Since interference with CA occurs with even fixed sporocysts, the effect is not due to IgG stimulated modulation of the parasite surface. Using two antisera with some overlapping specificities, and quantitative immunofluorescent antibody technique (QIFAT), we determined the concentrations of IgG needed to place equivalent amounts of IgG on the sporocysts. At these concentrations, CA was affected differentially, implying that interference was due to the specific antigens bound, and not due simply to the presence of IgG. Also with QIFAT we determined how much F(ab')2 and IgG from anti-sporocyst serum were needed to block an equivalent amount of antigenic determinants from access by whole FITC labelled IgG. Sporocysts whose surface antigens were equally blocked were equally unadherent for hemocytes, supporting the notion that the nature of obscured antigens, and neither the Fc portion nor the larger size of intact IgG protein, was responsible for the effect on CA. These surprising results imply a role for specific antigen binding sites on snail hemocytes.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1111/j.1365-3024.1984.tb00822.xDOIArticle
http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3024.1984.tb00822.x/abstractPublisherArticle
ORCID:
AuthorORCID
Yui, Mary A.0000-0002-3136-2181
Additional Information:© 1984, John Wiley and Sons. Accepted for publication 24 May 1984.
Issue or Number:1
Record Number:CaltechAUTHORS:20170303-165004725
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170303-165004725
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:74751
Collection:CaltechAUTHORS
Deposited By: Donna Wrublewski
Deposited On:17 Mar 2017 21:23
Last Modified:03 Oct 2019 16:42

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