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RNA-Seq and find: entering the RNA deep field

Roberts, Adam and Pachter, Lior (2011) RNA-Seq and find: entering the RNA deep field. Genome Medicine, 3 (11). Art. No. 74. ISSN 1756-994X. PMCID PMC3308029. doi:10.1186/gm290. https://resolver.caltech.edu/CaltechAUTHORS:20170306-093511106

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Abstract

Initial high-throughput RNA sequencing (RNA-Seq) experiments have revealed a complex and dynamic transcriptome, but because it samples transcripts in proportion to their abundances, assessing the extent and nature of low-level transcription using this technique has been difficult. A new assay, RNA CaptureSeq, addresses this limitation of RNA-Seq by enriching for low-level transcripts with cDNA tiling arrays prior to high-throughput sequencing. This approach reveals a plethora of transcripts that have been previously dismissed as 'noise', and hints at single-cell transcription fingerprints that may be crucial in defining cellular function in normal and disease states.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1186/gm290DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3308029/PubMed CentralArticle
ORCID:
AuthorORCID
Pachter, Lior0000-0002-9164-6231
Additional Information:© 2011 BioMed Central Ltd. Published: 22 November 2011. AR is supported by a graduate research fellowship from the National Science Foundation. LP is supported in part by grant NIH R01 HG006129-01. We thank Meromit Singer for comments on the curse of deep sequencing. The authors declare that they have no competing interests.
Funders:
Funding AgencyGrant Number
NSF Graduate Research FellowshipUNSPECIFIED
NIHR01 HG006129-01
Issue or Number:11
PubMed Central ID:PMC3308029
DOI:10.1186/gm290
Record Number:CaltechAUTHORS:20170306-093511106
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170306-093511106
Official Citation:Roberts A, Pachter L: RNA-Seq and find: entering the RNA deep field. Genome Medicine 2011, 3:74.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:74766
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:06 Mar 2017 17:59
Last Modified:11 Nov 2021 05:29

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