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Flow effects on the viability and lysis of suspended mammalian cells

McQueen, Anne and Meilhoc, Eliane and Bailey, James E. (1987) Flow effects on the viability and lysis of suspended mammalian cells. Biotechnology Letters, 9 (12). pp. 831-836. ISSN 0141-5492. https://resolver.caltech.edu/CaltechAUTHORS:20170306-101145611

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Abstract

A mouse myeloma cell line growing in suspension was subjected intermittently to flow through a sudden contraction and turbulent flow in a capillary tube. The probability of lysis per pass through the capillary tube increased with average wall shear stress level and with residence time per pass in the tube. Lysis was first observed at a threshold average wall shear stress level of 1800 dyn/cm^2. Although the flow caused lysis, it had no effect on cell viability.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1007/BF01026191DOIArticle
http://link.springer.com/article/10.1007%2FBF01026191PublisherArticle
Additional Information:© 1987 Science and Technology Letters. First Online: 01 December 1987.
Issue or Number:12
Record Number:CaltechAUTHORS:20170306-101145611
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170306-101145611
Official Citation:McQueen, A., Meilhoc, E. & Bailey, J.E. Biotechnol Lett (1987) 9: 831. doi:10.1007/BF01026191
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:74771
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:06 Mar 2017 18:19
Last Modified:03 Oct 2019 16:42

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