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Copper(II) Binding to α-Synuclein, the Parkinson’s Protein

Lee, Jennifer C. and Gray, Harry B. and Winkler, Jay R. (2008) Copper(II) Binding to α-Synuclein, the Parkinson’s Protein. Journal of the American Chemical Society, 130 (22). pp. 6898-6899. ISSN 0002-7863. PMCID PMC2664836. doi:10.1021/ja711415b. https://resolver.caltech.edu/CaltechAUTHORS:20170306-150523974

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Abstract

Variations in tryptophan fluorescence intensities confirm that copper(II) interacts with α-synuclein, a protein implicated in Parkinson’s disease. Trp4 fluorescence decay kinetics measured for the F4W protein show that Cu(II) binds tightly (K_d ∼ 100 nM) near the N-terminus at pH 7. Work on a F4W/H50S mutant indicates that a histidine imidazole is not a ligand in this high-affinity site.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja711415bDOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja711415bPublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ja711415bPublisherSupporting Information
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2664836/PubMed CentralArticle
ORCID:
AuthorORCID
Gray, Harry B.0000-0002-7937-7876
Winkler, Jay R.0000-0002-4453-9716
Additional Information:© 2008 American Chemical Society. ACS AuthorChoice. Received December 26, 2007; Publication Date (Web): May 9, 2008. Supported by the Intramural Research Program of the National Institutes of Health, the National Heart, Lung, and Blood Institute (JCL), and grants from the Ellison Medical Foundation (Senior Scholar Award in Aging to H.B.G.) and NIH (GM068461 to J.R.W.; DK19038 to H.B.G.). Initial work was supported by a Beckman Senior Research Fellowship (J.C.L.).
Funders:
Funding AgencyGrant Number
Ellison Medical FoundationUNSPECIFIED
NIHGM068461
NIHDK19038
Arnold and Mabel Beckman FoundationUNSPECIFIED
Issue or Number:22
PubMed Central ID:PMC2664836
DOI:10.1021/ja711415b
Record Number:CaltechAUTHORS:20170306-150523974
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170306-150523974
Official Citation:Copper(II) Binding to α-Synuclein, the Parkinson’s Protein Jennifer C. Lee, Harry B. Gray, and Jay R. Winkler Journal of the American Chemical Society 2008 130 (22), 6898-6899 DOI: 10.1021/ja711415b
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:74809
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:07 Mar 2017 00:10
Last Modified:11 Nov 2021 05:30

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