Copper(II) Binding to α-Synuclein, the Parkinson’s Protein

Lee, Jennifer C. and Gray, Harry B. and Winkler, Jay R. (2008) Copper(II) Binding to α-Synuclein, the Parkinson’s Protein. Journal of the American Chemical Society, 130 (22). pp. 6898-6899. ISSN 0002-7863. PMCID PMC2664836. doi:10.1021/ja711415b. https://resolver.caltech.edu/CaltechAUTHORS:20170306-150523974

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Abstract

Variations in tryptophan fluorescence intensities confirm that copper(II) interacts with α-synuclein, a protein implicated in Parkinson’s disease. Trp4 fluorescence decay kinetics measured for the F4W protein show that Cu(II) binds tightly (K_d ∼ 100 nM) near the N-terminus at pH 7. Work on a F4W/H50S mutant indicates that a histidine imidazole is not a ligand in this high-affinity site.

Item Type:

Article

Related URLs:

URL	URL Type	Description
http://dx.doi.org/10.1021/ja711415b	DOI	Article
http://pubs.acs.org/doi/abs/10.1021/ja711415b	Publisher	Article
http://pubs.acs.org/doi/suppl/10.1021/ja711415b	Publisher	Supporting Information
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2664836/	PubMed Central	Article

ORCID:

Author	ORCID
Gray, Harry B.	0000-0002-7937-7876
Winkler, Jay R.	0000-0002-4453-9716

Additional Information:

© 2008 American Chemical Society. ACS AuthorChoice. Received December 26, 2007; Publication Date (Web): May 9, 2008. Supported by the Intramural Research Program of the National Institutes of Health, the National Heart, Lung, and Blood Institute (JCL), and grants from the Ellison Medical Foundation (Senior Scholar Award in Aging to H.B.G.) and NIH (GM068461 to J.R.W.; DK19038 to H.B.G.). Initial work was supported by a Beckman Senior Research Fellowship (J.C.L.).

Funders:

Funding Agency	Grant Number
Ellison Medical Foundation	UNSPECIFIED
NIH	GM068461
NIH	DK19038
Arnold and Mabel Beckman Foundation	UNSPECIFIED

Issue or Number:

PubMed Central ID:

PMC2664836

DOI:

10.1021/ja711415b

Record Number:

CaltechAUTHORS:20170306-150523974

Persistent URL:

https://resolver.caltech.edu/CaltechAUTHORS:20170306-150523974

Official Citation:

Copper(II) Binding to α-Synuclein, the Parkinson’s Protein Jennifer C. Lee, Harry B. Gray, and Jay R. Winkler Journal of the American Chemical Society 2008 130 (22), 6898-6899 DOI: 10.1021/ja711415b

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ID Code:

74809

Collection:

CaltechAUTHORS

Deposited By:

Tony Diaz

Deposited On:

07 Mar 2017 00:10

Last Modified:

11 Nov 2021 05:30

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