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Acoustic Behavior of Halobacterium salinarum Gas Vesicles in the High-Frequency Range: Experiments and Modeling

Cherin, Emmanuel and Melis, Johan M. and Bourdeau, Raymond W. and Yin, Melissa and Kochmann, Dennis M. and Foster, F. Stuart and Shapiro, Mikhail G. (2017) Acoustic Behavior of Halobacterium salinarum Gas Vesicles in the High-Frequency Range: Experiments and Modeling. Ultrasound in Medicine and Biology, 43 (5). pp. 1016-1030. ISSN 0301-5629. PMCID PMC5385285. https://resolver.caltech.edu/CaltechAUTHORS:20170313-090300487

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Abstract

Gas vesicles (GVs) are a new and unique class of biologically derived ultrasound contrast agents with sub-micron size whose acoustic properties have not been fully elucidated. In this study, we investigated the acoustic collapse pressure and behavior of Halobacterium salinarum gas vesicles at transmit center frequencies ranging from 12.5 to 27.5 MHz. The acoustic collapse pressure was found to be above 550 kPa at all frequencies, nine-fold higher than the critical pressure observed under hydrostatic conditions. We illustrate that gas vesicles behave non-linearly when exposed to ultrasound at incident pressure ranging from 160 kPa to the collapse pressure and generate second harmonic amplitudes of −2 to −6 dB below the fundamental in media with viscosities ranging from 0.89 to 8 mPa·s. Simulations performed using a Rayleigh–Plesset-type model accounting for buckling and a dynamic finite-element analysis suggest that buckling is the mechanism behind the generation of harmonics. We found good agreement between the level of second harmonic relative to the fundamental measured at 20 MHz and the Rayleigh–Plesset model predictions. Finite-element simulations extended these findings to a non-spherical geometry, confirmed that the acoustic buckling pressure corresponds to the critical pressure under hydrostatic conditions and support the hypothesis of limited gas flow across the GV shell during the compression phase in the frequency range investigated. From simulations, estimates of GV bandwidth-limited scattering indicate that a single GV has a scattering cross section comparable to that of a red blood cell. These findings will inform the development of GV-based contrast agents and pulse sequences to optimize their detection with ultrasound.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.ultrasmedbio.2016.12.020DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5385285PubMed CentralArticle
ORCID:
AuthorORCID
Bourdeau, Raymond W.0000-0003-2202-1980
Kochmann, Dennis M.0000-0002-9112-6615
Shapiro, Mikhail G.0000-0002-0291-4215
Additional Information:© 2017 World Federation for Ultrasound in Medicine & Biology. Received 3 March 2016, Revised 20 December 2016, Accepted 24 December 2016, Available online 1 March 2017. We thank Dave Goertz for insightful discussions regarding microbubble dynamic and modeling, Anupama Lakshmanan and Suchita Nety for advice on GV and phantom preparation, Jordan Dykes for building the hydrostatic collapse measurement system and Flavien Saenz Molina for assistance with FEM. This research was supported by the Canadian Institute of Health Research (MOP: 136842), the National Institute of Health (1R01EB018975), the Burroughs Wellcome Career Award at the Scientific Interface and FUJIFILM VisualSonics.
Funders:
Funding AgencyGrant Number
Canadian Institutes of Health Research (CIHR)MOP-136842
NIH1R01EB018975
Burroughs Wellcome FundUNSPECIFIED
FUJIFILM VisualSonicsUNSPECIFIED
Subject Keywords:Submicron gas vesicles; Contrast agent; High-frequency ultrasound; Buckling; Modeling
Issue or Number:5
PubMed Central ID:PMC5385285
Record Number:CaltechAUTHORS:20170313-090300487
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170313-090300487
Official Citation:Emmanuel Cherin, Johan M. Melis, Raymond W. Bourdeau, Melissa Yin, Dennis M. Kochmann, F. Stuart Foster, Mikhail G. Shapiro, Acoustic Behavior of Halobacterium salinarum Gas Vesicles in the High-Frequency Range: Experiments and Modeling, Ultrasound in Medicine & Biology, Volume 43, Issue 5, May 2017, Pages 1016-1030, ISSN 0301-5629, https://doi.org/10.1016/j.ultrasmedbio.2016.12.020. (http://www.sciencedirect.com/science/article/pii/S0301562917300066)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:75050
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:13 Mar 2017 16:11
Last Modified:25 Nov 2019 18:02

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