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Targeted mutagenesis and genetic analysis of a Drosophila receptor-linked protein tyrosine phosphatase gene

Hamilton, B. A. and Ho, A. and Zinn, Kai (1995) Targeted mutagenesis and genetic analysis of a Drosophila receptor-linked protein tyrosine phosphatase gene. Roux's Archives of Developmental Biology, 204 (3). pp. 187-192. ISSN 0930-035X. doi:10.1007/BF00241271.

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Several Drosophila receptor-linked protein tyrosine phosphatases (R-PTPs) are selectively expressed on axons of the developing embryonic central nervous system. The extracellular domains of these axonal R-PTPs are homologous to neural adhesion molecules. Thus, R-PTPs may directly couple cell recognition to signal transduction via control of tyrosine phosphorylation. To examine the function of these molecules during nervous system development, we wished to generate mutations in R-PTP genes. It was unclear whether a mutation in a single R-PTP gene would confer lethality, however, because the similarities in sequence and expression pattern between the axonal R-PTPs suggest that they may have partially redundant functions. To circumvent this problem, we developed a directed mutagenesis strategy based on local transposition of P elements, and used this approach to isolate a null mutation in the DPTP99A gene. This strategy, which we describe in detail here, should be applicable to any Drosophila gene within a lettered division of an appropriately marked P element. Flies lacking DPTP99A expression are viable and fertile, and we have been unable to detect any alterations in the embryonic nervous system of DPTP99A embryos using a variety of antibody markers.

Item Type:Article
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Zinn, Kai0000-0002-6706-5605
Additional Information:© 1995 Springer Verlag. Received: 30 May 1994; Accepted: 13 July 1994.
Subject Keywords:Targeted mutagenesis; Pelement-local transposition; Protein tyrosine phosphatase; Drosophila embryonic nervous system
Issue or Number:3
Record Number:CaltechAUTHORS:20170320-141926627
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Official Citation:Hamilton, B.A., Ho, A. & Zinn, K. Roux's Arch Dev Biol (1995) 204: 187. doi:10.1007/BF00241271
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:75250
Deposited By: Tony Diaz
Deposited On:20 Mar 2017 22:28
Last Modified:15 Nov 2021 16:32

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