Tezcan, F. Akif and Winkler, Jay R. and Gray, Harry B. (1999) Probing Protein Folding with Substitution-Inert Metal Ions. Folding Kinetics of Cobalt(III)-Cytochrome c. Journal of the American Chemical Society, 121 (50). pp. 11918-11919. ISSN 0002-7863. doi:10.1021/ja993447k. https://resolver.caltech.edu/CaltechAUTHORS:20170322-152801776
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Abstract
Ligand-substitution processes at the heme strongly influence peptide backbone dynamics during the folding of cytochrome c (cyt c). When cyt c is unfolded with guanidine hydrochloride (GuHCl) at pH 7, one of the axial ligands (Met 80) is replaced by a nitrogenous base from an amino acid residue; this misligation introduces an energy barrier with an associated folding time of several hundred milliseconds. A great deal of evidence points to His 26 or His 33 as the ligand in unfolded horse heart cyt c. Nevertheless, recent studies indicate that other bases (Lys or N-terminus in yeast cyt c) can act as ligands as well. We have found that the substitution-inert heme in the Co(III) derivative of cyt c (Co-cyt c) allows a closer look at the folding kinetics and the ligands in the unfolded form of this protein.
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| Additional Information: | © 1999 American Chemical Society. Received September 23, 1999. Publication Date (Web): December 3, 1999. We thank Ivano Bertini, Kara Bren, and Paola Turano for helpful discussions. This work was supported by NSF (MCB-9974477) and the Arnold and Mabel Beckman Foundation. | |||||||||
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| Issue or Number: | 50 | |||||||||
| DOI: | 10.1021/ja993447k | |||||||||
| Record Number: | CaltechAUTHORS:20170322-152801776 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20170322-152801776 | |||||||||
| Official Citation: | Probing Protein Folding with Substitution-Inert Metal Ions. Folding Kinetics of Cobalt(III)-Cytochrome c F. Akif Tezcan, Jay R. Winkler, and Harry B. Gray Journal of the American Chemical Society 1999 121 (50), 11918-11919 DOI: 10.1021/ja993447k | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 75324 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | George Porter | |||||||||
| Deposited On: | 23 Mar 2017 20:10 | |||||||||
| Last Modified: | 15 Nov 2021 16:32 |
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