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Primary in vitro stimulation of antibody production by rainbow trout lymphocytes

Kaattari, S. L. and Irwin, M. J. and Yui, M. A. and Tripp, R. A. and Parkins, J. S. (1986) Primary in vitro stimulation of antibody production by rainbow trout lymphocytes. Veterinary Immunology and Immunopathology, 12 (1-4). pp. 29-38. ISSN 0165-2427. https://resolver.caltech.edu/CaltechAUTHORS:20170324-085913382

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Abstract

Trinitrophenylated (TNP) forms ofE. coli lipopolysaccharide (LPS) and keyhole limpet hemocyanin (KLH) were used to produce antigen specific plaque-forming cell (PFC) responses with rainbow trout (Salmo gairdneri) splenocytes from unprimed fishin vitro. The culture system that was developed is described and characterized with respect to the kinetics and dose responses for both the haptenated and unhaptenated forms of the carriers. The induction of the PFC response to TNP-LPS was inhibited with TNP-lysine. Exposure to graded levels of gamma-radiation demonstrated a low dose augmentation of the PFC response with both antigens. Antigen addition experiments reveal that both antigens appear to stimulate the same population of antibody-producing B lymphocytes.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/0165-2427(86)90107-8DOIArticle
http://www.sciencedirect.com/science/article/pii/0165242786901078PublisherArticle
ORCID:
AuthorORCID
Yui, M. A.0000-0002-3136-2181
Additional Information:© 1986 Elsevier B.V.
Issue or Number:1-4
Record Number:CaltechAUTHORS:20170324-085913382
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170324-085913382
Official Citation:S.L. Kaattari, M.J. Irwin, M.A. Yui, R.A. Tripp, J.S. Parkins, Primary in vitro stimulation of antibody production by rainbow trout lymphocytes, Veterinary Immunology and Immunopathology, Volume 12, Issue 1, 1986, Pages 29-38, ISSN 0165-2427, http://dx.doi.org/10.1016/0165-2427(86)90107-8. (http://www.sciencedirect.com/science/article/pii/0165242786901078)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:75380
Collection:CaltechAUTHORS
Deposited By: Donna Wrublewski
Deposited On:24 Mar 2017 16:11
Last Modified:03 Oct 2019 16:49

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