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The Chicken Yolk Sac IgY Receptor, a Functional Equivalent of the Mammalian MHC-Related Fc Receptor, Is a Phospholipase A_2 Receptor Homolog

West, Anthony P., Jr. and Herr, Andrew B. and Bjorkman, Pamela J. (2004) The Chicken Yolk Sac IgY Receptor, a Functional Equivalent of the Mammalian MHC-Related Fc Receptor, Is a Phospholipase A_2 Receptor Homolog. Immunity, 20 (5). pp. 601-610. ISSN 1074-7613. doi:10.1016/S1074-7613(04)00113-X. https://resolver.caltech.edu/CaltechAUTHORS:20170327-141359020

[img] PDF (Figure S1 - Alignment of the Predicted Amino Acid Sequence of FcRY with Human PLA2R and Human MR) - Supplemental Material
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[img] Image (JPEG) (Figure S2 - Staining of FcRY-Transfected CHO Cells by Cy3-Labeled FcY at pH 6.0) - Supplemental Material
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[img] Image (JPEG) (Figure S3 - Analytical Ultracentrifugation Sedimentation Equilibrium Analysis of FcRY at (A) pH 8.0 and (B) pH 6.0 at Three Speeds) - Supplemental Material
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[img] PDF (Supplemental Data) - Supplemental Material
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Abstract

In mammals, IgG is transferred from mother to young by the MHC-related receptor FcRn, which binds IgG in acidic endosomes and releases it at basic pH into blood. Maternal IgY, the avian counterpart of IgG, is transferred to embryos across yolk sac membranes. We affinity-purified the chicken yolk sac IgY receptor (FcRY) and sequenced its gene. FcRY is unrelated to MHC molecules but is a homolog of the mammalian phospholipase A_2 receptor. Analytical ultracentrifugation and truncation experiments suggest that FcRY forms a compact structure containing an IgY binding site at acidic pH but undergoes a conformational change at basic pH that disrupts the site. FcRY is thus unrelated to mammalian FcRn in both its structure and mechanism for pH-dependent binding, illustrating distinct routes utilized by evolution to transfer antibodies.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S1074-7613(04)00113-XDOIArticle
http://www.sciencedirect.com/science/article/pii/S107476130400113XPublisherArticle
ORCID:
AuthorORCID
Bjorkman, Pamela J.0000-0002-2277-3990
Additional Information:© 2004 Cell Press. Received 1 January 2004, Revised 1 March 2004, Accepted 17 March 2004, Available online 18 May 2004Published: May 18, 2004. We thank Gary M. Hathaway from the Caltech protein and peptide microanalysis facility for performing the protein microsequencing, digestion, peptide HPLC, and mass spectroscopy; Peter Snow of the Caltech Protein Expression Center for baculovirus expression of FcRY; Marianne Bronner-Fraser for providing fertilized chicken eggs; Marta Murphy for cover artwork; and members of the Bjorkman laboratory for critical reading of the manuscript. This work was supported by the Cancer Research Fund of the Damon-Runyon Walter Winchell Foundation (DRG-1445 to A.P.W. and DRG-1658 to A.B.H.), a career research award from the Burroughs-Wellcome Fund (A.P.W.), the NIH (2 R01 AI041239-06A1 to P.J.B.), and the Howard Hughes Medical Institute (P.J.B.). Accession Numbers: The FcRY cDNA sequence has been deposited in GenBank with accession number AY450642.
Funders:
Funding AgencyGrant Number
Damon Runyon-Walter Winchell Foundation Cancer Research FundDRG-1445
Damon Runyon-Walter Winchell Foundation Cancer Research FundDRG-1658
Burroughs-Wellcome FundUNSPECIFIED
NIH2 R01 AI041239-06A1
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Issue or Number:5
DOI:10.1016/S1074-7613(04)00113-X
Record Number:CaltechAUTHORS:20170327-141359020
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170327-141359020
Official Citation:Anthony P West Jr., Andrew B Herr, Pamela J Bjorkman, The Chicken Yolk Sac IgY Receptor, a Functional Equivalent of the Mammalian MHC-Related Fc Receptor, Is a Phospholipase A2 Receptor Homolog, Immunity, Volume 20, Issue 5, May 2004, Pages 601-610, ISSN 1074-7613, http://dx.doi.org/10.1016/S1074-7613(04)00113-X. (http://www.sciencedirect.com/science/article/pii/S107476130400113X)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:75431
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:27 Mar 2017 22:21
Last Modified:15 Nov 2021 16:33

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