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Leukocyte count and two-part differential in whole blood based on a portable microflow cytometer

Shi, W. and Zheng, S. and Kasdan, H. L. and Fridge, A. and Tai, Y. C. (2009) Leukocyte count and two-part differential in whole blood based on a portable microflow cytometer. In: TRANSDUCERS 2009 - 2009 International Solid-State Sensors, Actuators and Microsystems Conference. IEEE , Piscataway, NJ, pp. 616-619. ISBN 978-1-4244-4190-7. https://resolver.caltech.edu/CaltechAUTHORS:20170328-174508653

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Abstract

This work demonstrated leukocyte count and two-part leukocyte differential from the whole blood based on a portable microflow cytometer system. Leukocytes are selectively stained with a fluorescent dye, Acridine Orange (AO). The blood sample is then pumped through a disposable microfluidic device for fluorescence sensing. Under blue LED excitation (460nm), the green fluorescence from DNA and the red fluorescence from RNA are detected simultaneously with two photomultiplier tubes (PMT). Leukocytes are counted and differentiated into two parts, lymphocyte versus non-lymphocyte, based on their fluorescence signatures. The results, including leukocyte absolute count and lymphocyte ratio, matched well with the commercial hemacytometer counts (maximal error 9.0%, correlation coefficient ~0.8). We also tested the system for the first time under a zero-gravity (zero-G) environment that facilitated its application in space missions.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1109/SENSOR.2009.5285400DOIArticle
http://ieeexplore.ieee.org/document/5285400/PublisherArticle
ORCID:
AuthorORCID
Tai, Y. C.0000-0001-8529-106X
Additional Information:© 2009 IEEE. This work was supported by NSBRI/NASA under the Award Number TD01301. The authors especially thank Shannon Melton and Victor Hurst IV from Wyle Integrated Science and Engineering for their help on zero-G flight test. The authors also wish to thank all members of Caltech Micromachining Group for helpful discussions.
Funders:
Funding AgencyGrant Number
National Space Biomedical Research Institute (NSBRI)TD01301
Subject Keywords:Leukocyte, Count, Differential, Fluorescence, Acridine Orange
Record Number:CaltechAUTHORS:20170328-174508653
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170328-174508653
Official Citation:W. Shi, S. Zheng, H. L. Kasdan, A. Fridge and Y. C. Tai, "Leukocyte count and two-part differential in whole blood based on a portable microflow cytometer," TRANSDUCERS 2009 - 2009 International Solid-State Sensors, Actuators and Microsystems Conference, Denver, CO, 2009, pp. 616-619. doi: 10.1109/SENSOR.2009.5285400
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:75497
Collection:CaltechAUTHORS
Deposited By: Kristin Buxton
Deposited On:29 Mar 2017 00:56
Last Modified:03 Oct 2019 16:51

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