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Electron-microscopic visualization of deletion mutations

Davis, Ronald W. and Davidson, Norman (1968) Electron-microscopic visualization of deletion mutations. Proceedings of the National Academy of Sciences of the United States of America, 60 (1). pp. 243-250. ISSN 0027-8424. doi:10.1073/pnas.60.1.243.

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Deletion mutants of λ coliphage were discovered by Kellenberger, Zichichi, and Weigle.(1) The deletions can be mapped by genetic recombination experiments. It is not known if the recombination maps thus obtained give the true physical position of the deletions. The present paper describes a method for mapping deletion mutations with the electron microscope, thus obtaining the physical position of the deletion.

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Additional Information:© 1968 by the National Academy of Sciences. Communicated February 12, 1968. We are grateful to Dr. Robert Huskey for his help and phage stocks, to Dr. James G. Wetmur for advice on annealing conditions, and to Dr. Jean Weigle for his patient guidance and counsel in many matters. This work has been supported by research grant GM 10991 and training grant GM 1262 from the U.S. Public Health Service.
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7564
Deposited By: Tony Diaz
Deposited On:06 Mar 2007
Last Modified:08 Nov 2021 20:43

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