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A Method for Photoinitating Protein Folding in a Nondenaturing Environment

Hansen, Kirk C. and Rock, Ronald S. and Larsen, Randy W. and Chan, Sunney I. (2000) A Method for Photoinitating Protein Folding in a Nondenaturing Environment. Journal of the American Chemical Society, 122 (46). pp. 11567-11568. ISSN 0002-7863. http://resolver.caltech.edu/CaltechAUTHORS:20170404-104411405

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Abstract

The early kinetic events of protein folding are an important part of the folding pathway, yet our understanding towards the process is limited. Information from the study of these early events can allow us to distinguish between the various models that have been proposed to describe the folding of a protein in real time. Unlike “typical” chemical kinetics with well-defined initial and final states, the initial state of a denatured protein is relatively ill-defined. This uncertainty introduces ambiguity in the interpretation of the experimental data on the early events in protein folding. Toward developing a unified theory of protein folding, it is necessary to begin the observation of the refolding process from a well-defined initial state, trigger folding as rapidly as possible, and to follow the protein in real time as it samples its conformational space over its highly complex free-energy landscape.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja002949rDOIArticle
http://pubs.acs.org/doi/full/10.1021/ja002949rPublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ja002949rRelated ItemSupporting Information
ORCID:
AuthorORCID
Chan, Sunney I.0000-0002-5348-2723
Additional Information:© 2000 American Chemical Society. Received 10 August 2000. Published online 5 November 2000. Published in print 1 November 2000. Funding for this project came from Grant GM22432 from the National Institutes of Health and from the National Science Foundation, MCB9904713 (R.W.L.). K.C.H. and R.S.R. are the recipients of National Research Service Predoctoral Awards.
Funders:
Funding AgencyGrant Number
NIHGM-22432
NSFMCB-9904713
NIH Predoctoral FellowshipUNSPECIFIED
Record Number:CaltechAUTHORS:20170404-104411405
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20170404-104411405
Official Citation:A Method for Photoinitating Protein Folding in a Nondenaturing Environment Kirk C. Hansen, Ronald S. Rock, Randy W. Larsen, and Sunney I. Chan Journal of the American Chemical Society 2000 122 (46), 11567-11568 DOI: 10.1021/ja002949r
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:75691
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:04 Apr 2017 18:46
Last Modified:24 Jul 2018 22:30

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