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Local Retinal Circuits of Melanopsin-Containing Ganglion Cells Identified by Transsynaptic Viral Tracing

Viney, Tim James and Balint, Kamill and Hillier, Daniel and Siegert, Sandra and Boldogkoi, Zsolt and Enquist, Lynn W. and Meister, Markus and Cepko, Constance L. and Roska, Botond (2007) Local Retinal Circuits of Melanopsin-Containing Ganglion Cells Identified by Transsynaptic Viral Tracing. Current Biology, 17 (11). pp. 981-988. ISSN 0960-9822. doi:10.1016/j.cub.2007.04.058. https://resolver.caltech.edu/CaltechAUTHORS:20170404-140543270

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Abstract

Intrinsically photosensitive melanopsin-containing retinal ganglion cells (ipRGCs) control important physiological processes, including the circadian rhythm, the pupillary reflex, and the suppression of locomotor behavior (reviewed in [1]). ipRGCs are also activated by classical photoreceptors, the rods and cones, through local retinal circuits 2 ; 3. ipRGCs can be transsynaptically labeled through the pupillary-reflex circuit with the derivatives of the Bartha strain of the alphaherpesvirus pseudorabies virus(PRV) 4 ; 5 that express GFP 6; 7; 8; 9; 10; 11 ; 12. Bartha-strain derivatives spread only in the retrograde direction [13]. There is evidence that infected cells function normally for a while during GFP expression [7]. Here we combine transsynaptic PRV labeling, two-photon laser microscopy, and electrophysiological techniques to trace the local circuit of different ipRGC subtypes in the mouse retina and record light-evoked activity from the transsynaptically labeled ganglion cells. First, we show that ipRGCs are connected by monostratified amacrine cells that provide strong inhibition from classical-photoreceptor-driven circuits. Second, we show evidence that dopaminergic interplexiform cells are synaptically connected to ipRGCs. The latter finding provides a circuitry link between light–dark adaptation and ipRGC function.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.cub.2007.04.058DOIArticle
ORCID:
AuthorORCID
Meister, Markus0000-0003-2136-6506
Additional Information:© 2007 Elsevier Under an Elsevier user license. Received 11 January 2007, Revised 28 April 2007, Accepted 30 April 2007, Available online 24 May 2007Published online: May 24, 2007. We are grateful for the assistance of Z. Springer and B. Gross Scherf. We thank F. Albeanu for his advice on building the two-photon microscope, F. Engert for providing us with the imaging software Tango, and P. Caroni for his comments on the paper. This study was supported by Office of Naval Research Multidisciplinary University Research Initiative [ONR MURI] and Naval International Cooperative Opportunities in Science and Technology Program [NICOP] grants, a Marie Curie Excellence Grant, a Human Frontier Science Program [HFSP] Young Investigator grant, and Friedrich Miescher Institute funds to B.R. I. Provencio kindly provided the melanopsin antibody. B.W. Banfield kindly provided PRV 614. Viral infections, electrophysiological recordings, and immunohistochemistry have been done at both Harvard and the Friedrich Miescher Institute. Two-photon time-lapse imaging, automatic confocal scanning, and automated image analysis were done at the Friedrich Miescher Institute. Membrane-bound-GFP-expressing PRV was developed at University of Szeged.
Funders:
Funding AgencyGrant Number
Office of Naval Research (ONR)UNSPECIFIED
Naval International Cooperative Opportunities in Science and Technology Program (NICOP)UNSPECIFIED
Marie Curie FellowshipUNSPECIFIED
Human Frontier Science ProgramUNSPECIFIED
Friedrich Miescher InstituteUNSPECIFIED
Subject Keywords:SYSNEURO
Issue or Number:11
DOI:10.1016/j.cub.2007.04.058
Record Number:CaltechAUTHORS:20170404-140543270
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170404-140543270
Official Citation:Tim James Viney, Kamill Balint, Daniel Hillier, Sandra Siegert, Zsolt Boldogkoi, Lynn W. Enquist, Markus Meister, Constance L. Cepko, Botond Roska, Local Retinal Circuits of Melanopsin-Containing Ganglion Cells Identified by Transsynaptic Viral Tracing, Current Biology, Volume 17, Issue 11, 5 June 2007, Pages 981-988, ISSN 0960-9822, http://dx.doi.org/10.1016/j.cub.2007.04.058. (http://www.sciencedirect.com/science/article/pii/S0960982207013395)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:75701
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:04 Apr 2017 21:35
Last Modified:15 Nov 2021 16:35

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