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γ-Protocadherins regulate neuronal survival but are dispensable for circuit formation in retina

Lefebvre, Julie L. and Zhang, Yifeng and Meister, Markus and Wang, Xiaozhong and Sanes, Joshua R. (2008) γ-Protocadherins regulate neuronal survival but are dispensable for circuit formation in retina. Development, 135 (24). pp. 4141-4151. ISSN 0950-1991. PMCID PMC2644426. http://resolver.caltech.edu/CaltechAUTHORS:20170404-141850023

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[img] PDF (Fig. S2. Synaptic puncta density is similar in Pcdh-γ; Bax double mutant and control retinas) - Supplemental Material
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Abstract

Twenty-two tandemly arranged protocadherin-γ (Pcdh-γ) genes encode transmembrane proteins with distinct cadherin-related extracellular domains and a common intracellular domain. Genetic studies have implicated Pcdh-γ genes in the regulation of neuronal survival and synapse formation. Because mice lacking the Pcdh-γ cluster die perinatally, we generated conditional mutants to analyze roles of Pcdh-γ genes in the development and function of neural circuits. Retina-specific deletion of Pcdh-γs led to accentuation of naturally occurring death of interneurons and retinal ganglion cells (RGCs) during the first two postnatal weeks. Nonetheless, many neuronal subtypes formed lamina-specific arbors. Blocking apoptosis by deletion of the pro-apoptotic gene Bax showed that even neurons destined to die formed qualitatively and quantitatively appropriate connections. Moreover, electrophysiological analysis indicated that processing of visual information was largely normal in the absence of Pcdh-γ genes. These results suggest that Pcdh-γ genes are dispensable for elaboration of specific connections in retina, but play a primary role in sculpting neuronal populations to appropriate sizes or proportions during the period of naturally occurring cell death.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1242/dev.027912DOIArticle
http://dev.biologists.org/content/135/24/4141PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2644426PubMed CentralArticle
http://dev.biologists.org/content/135/24/4141.supplementalPublisherSupplementary Information
ORCID:
AuthorORCID
Meister, Markus0000-0003-2136-6506
Alternate Title:Gamma Protocadherins regulate neuronal survival but are dispensable for circuit formation in retina
Additional Information:© 2008 Company of Biologists. Accepted September 24, 2008. We thank Joshua Weiner for sharing data. This work was supported by grants from the National Institutes of Health to M.M. and J.R.S, a NARSAD Young Investigator award to J.L., and Damon Runyon Cancer Research Foundation Fellowship to Y.-F.Z.
Funders:
Funding AgencyGrant Number
NIHUNSPECIFIED
Brain and Behavior Research FoundationUNSPECIFIED
Damon Runyon Cancer Research FoundationUNSPECIFIED
Subject Keywords:Apoptosis, Interneuron, Laminar specificity, Receptive field, Mouse
PubMed Central ID:PMC2644426
Record Number:CaltechAUTHORS:20170404-141850023
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20170404-141850023
Official Citation:γ-Protocadherins regulate neuronal survival but are dispensable for circuit formation in retina Julie L. Lefebvre, Yifeng Zhang, Markus Meister, Xiaozhong Wang, Joshua R. Sanes Development 2008 135: 4141-4151; doi: 10.1242/dev.027912
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:75702
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:04 Apr 2017 21:34
Last Modified:04 Apr 2017 21:34

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