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Retinal Ganglion Cells with Distinct Directional Preferences Differ in Molecular Identity, Structure, and Central Projections

Kay, Jeremy N. and De la Huerta, Irina and Kim, In-Jung and Zhang, Yifeng and Yamagata, Masahito and Chu, Monica W. and Meister, Markus and Sanes, Joshua R. (2011) Retinal Ganglion Cells with Distinct Directional Preferences Differ in Molecular Identity, Structure, and Central Projections. Journal of Neuroscience, 31 (21). pp. 7753-7762. ISSN 0270-6474. PMCID PMC3108146. http://resolver.caltech.edu/CaltechAUTHORS:20170405-063019589

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Abstract

The retina contains ganglion cells (RGCs) that respond selectively to objects moving in particular directions. Individual members of a group of ON-OFF direction-selective RGCs (ooDSGCs) detect stimuli moving in one of four directions: ventral, dorsal, nasal, or temporal. Despite this physiological diversity, little is known about subtype-specific differences in structure, molecular identity, and projections. To seek such differences, we characterized mouse transgenic lines that selectively mark ooDSGCs preferring ventral or nasal motion as well as a line that marks both ventral- and dorsal-preferring subsets. We then used the lines to identify cell surface molecules, including Cadherin 6, CollagenXXVα1, and Matrix metalloprotease 17, that are selectively expressed by distinct subsets of ooDSGCs. We also identify a neuropeptide, CART (cocaine- and amphetamine-regulated transcript), that distinguishes all ooDSGCs from other RGCs. Together, this panel of endogenous and transgenic markers distinguishes the four ooDSGC subsets. Patterns of molecular diversification occur before eye opening and are therefore experience independent. They may help to explain how the four subsets obtain distinct inputs. We also demonstrate differences among subsets in their dendritic patterns within the retina and their axonal projections to the brain. Differences in projections indicate that information about motion in different directions is sent to different destinations.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1523/JNEUROSCI.0907-11.2011DOIArticle
http://www.jneurosci.org/content/31/21/7753.fullPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3108146/PubMed CentralArticle
ORCID:
AuthorORCID
Meister, Markus0000-0003-2136-6506
Additional Information:© 2011 the authors. Beginning six months after publication the Work will be made freely available to the public on SfN’s website to copy, distribute, or display under a Creative Commons Attribution 4.0 International (CC BY 4.0) license (https://creativecommons.org/licenses/by/4.0/). Received Feb. 19, 2011; accepted March 25, 2011. This work was supported by grants from the NIH to I.-J.K., M.M., and J.R.S., a grant from NIH to J.R.S., Collaborative Innovation Award 43667 from HHMI, and fellowships from the Life Sciences Research Foundation to J.N.K. and from the Charles A. King Trust to Y.Z. We thank Sara Haddad, Debbie Pelusi, and Laura Stoppel for assistance.
Funders:
Funding AgencyGrant Number
NIHUNSPECIFIED
Life Sciences Research FoundationUNSPECIFIED
Charles A. King TrustUNSPECIFIED
Howard Hughes Medical Institute (HHMI)43667
PubMed Central ID:PMC3108146
Record Number:CaltechAUTHORS:20170405-063019589
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20170405-063019589
Official Citation:Retinal Ganglion Cells with Distinct Directional Preferences Differ in Molecular Identity, Structure, and Central Projections Jeremy N. Kay, Irina De la Huerta, In-Jung Kim, Yifeng Zhang, Masahito Yamagata, Monica W. Chu, Markus Meister, Joshua R. Sanes Journal of Neuroscience 25 May 2011, 31 (21) 7753-7762; DOI: 10.1523/JNEUROSCI.0907-11.2011
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:75711
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:05 Apr 2017 16:18
Last Modified:05 Apr 2017 16:18

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