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A General Route for Post-Translational Cyclization of mRNA Display Libraries

Millward, Steven W. and Takahashi, Terry T. and Roberts, Richard W. (2005) A General Route for Post-Translational Cyclization of mRNA Display Libraries. Journal of the American Chemical Society, 127 (41). pp. 14142-14143. ISSN 0002-7863. doi:10.1021/ja054373h.

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Cyclic peptides are attractive scaffolds for the design of conformationally constrained molecular therapeutics. Previously, biological display libraries could only be cyclized via disulfide bonds, which are labile and can be reduced in an intracellular environment. In this paper, we construct high diversity, covalently cyclized mRNA display libraries (>10^(13) sequences) and analyze the cyclization reaction using MALDI-TOF MS and unnatural amino acid incorporation. Our route allows the extent of cyclization to be evaluated quantitatively and is broadly applicable to a variety of cyclization chemistries.

Item Type:Article
Related URLs:
URLURL TypeDescription Information
Roberts, Richard W.0000-0002-8587-5097
Additional Information:© 2005 American Chemical Society. Received July 1, 2005; Publication Date (Web): September 27, 2005. The authors would like to thank Niki Zacharias at Neurion Pharmaceuticals for the generous gift of pdCpA and α-hydroxy phenylalanine-dCA. This work was supported by NIH R01 GM60416, NIH Training Grant T32 GM08501, and the California Institute of Technology.
Funding AgencyGrant Number
NIHR01 GM60416
NIH Predoctoral FellowshipT32 GM08501
Issue or Number:41
Record Number:CaltechAUTHORS:20170405-144317686
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Official Citation:A General Route for Post-Translational Cyclization of mRNA Display Libraries Steven W. Millward, Terry T. Takahashi, and Richard W. Roberts Journal of the American Chemical Society 2005 127 (41), 14142-14143 DOI: 10.1021/ja054373h
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:75744
Deposited By: Tony Diaz
Deposited On:05 Apr 2017 21:48
Last Modified:15 Nov 2021 16:36

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