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Identification of critical IgG binding epitopes on the neonatal Fc receptor

Vaughn, Daniel E. and Milburn, Christina M. and Penny, David M. and Martin, W. Lance and Johnson, Jennifer L. and Bjorkman, Pamela J. (1997) Identification of critical IgG binding epitopes on the neonatal Fc receptor. Journal of Molecular Biology, 274 (4). pp. 597-607. ISSN 0022-2836. https://resolver.caltech.edu/CaltechAUTHORS:20170405-154230630

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Abstract

The neonatal Fc receptor (FcRn) binds maternal immunoglobulin G (IgG) during the acquisition of passive immunity by the fetus or newborn. FcRn also binds IgG and returns it to the bloodstream, thus protecting IgG from a default degradative pathway. Biosensor assays have been used to characterize the interaction of a soluble form of rat FcRn with IgG, and demonstrate that FcRn dimerization and immobilization are necessary to reproduce in vivo binding characteristics. Here, we report the identification of several FcRn amino acid substitutions that disrupt its affinity for IgG and examine the effect of alteration of residues at the FcRn dimer interface. The role of these amino acids is discussed in the context of the previously reported structures of rat FcRn and a complex of FcRn with the Fc portion of IgG.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1006/jmbi.1997.1388DOIArticle
http://www.sciencedirect.com/science/article/pii/S0022283697913887PublisherArticle
ORCID:
AuthorORCID
Bjorkman, Pamela J.0000-0002-2277-3990
Additional Information:© 1997 Academic Press Limited. Received 16 June 1997, Revised 25 August 1997, Accepted 29 August 1997. We thank Rochelle Diamond and the Caltech Flow Cytometry facility for help with FACS and Luis Sanchez and Zsuzsa Hamburger for critical reading of the manuscript. This work was supported by a grant from the NIH (AI/GM41239 to P. J. B.) and a Camille and Henry Dreyfuss Teacher Scholar Award (P. J. B.)
Funders:
Funding AgencyGrant Number
NIHAI/GM41239
Camille and Henry Dreyfus FoundationUNSPECIFIED
Subject Keywords:surface plasmon resonance; biosensor; structural binding site; functional epitope; binding free energy
Issue or Number:4
Record Number:CaltechAUTHORS:20170405-154230630
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170405-154230630
Official Citation:Daniel E Vaughn, Christina M Milburn, David M Penny, W.Lance Martin, Jennifer L Johnson, Pamela J Bjorkman, Identification of critical IgG binding epitopes on the neonatal Fc receptor1, Journal of Molecular Biology, Volume 274, Issue 4, 12 December 1997, Pages 597-607, ISSN 0022-2836, http://dx.doi.org/10.1006/jmbi.1997.1388. (http://www.sciencedirect.com/science/article/pii/S0022283697913887)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:75754
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:05 Apr 2017 22:49
Last Modified:03 Oct 2019 16:53

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