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Direct Assessment of MHC Class I Binding by Seven Ly49 Inhibitory NK Cell Receptors

Hanke, Thomas and Takizawa, Hisao and McMahon, Christopher W. and Busch, Dirk H. and Pamer, Eric G. and Miller, Joseph D. and Altman, John D. and Liu, Yang and Cado, Dragana and Lemonnier, Francois A. and Bjorkman, Pamela J. and Raulet, David H. (1999) Direct Assessment of MHC Class I Binding by Seven Ly49 Inhibitory NK Cell Receptors. Immunity, 11 (1). pp. 67-77. ISSN 1074-7613. doi:10.1016/S1074-7613(00)80082-5.

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Mouse NK cells express at least seven inhibitory Ly49 receptors. Here we employ a semiquantitative cell–cell adhesion assay as well as class I/peptide tetramers to provide a comprehensive analysis of specificities of Ly49 receptors for class I MHC molecules in eight MHC haplotypes. Different Ly49 receptors exhibited diverse binding properties. The degree of class I binding was related to the extent of functional inhibition. The tetramer studies demonstrated that neither glycosylation nor coreceptors were necessary for class I binding to Ly49 receptors and uncovered peptide-specific recognition by a Ly49 receptor. The results provide a foundation for interpreting and integrating many existing functional studies as well as for designing tests of NK cell development and self-tolerance.

Item Type:Article
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URLURL TypeDescription
Liu, Yang0000-0002-8155-9134
Bjorkman, Pamela J.0000-0002-2277-3990
Additional Information:© 1999 Cell Press. Received 8 March 1999, Revised 20 May 1999. We thank Peter Snow for production of the recombinant Dd from baculovirus, Vinay Kumar and Porunelloor Mathew for the cDNA library, Werner Held for Ly49A transgenic mice, Chern-sing Goh and Ann Lazar for technical assistance, and Russell Vance for critical reading of the manuscript. T. H. was a recipient of a Research Fellowship from the Deutsche Forschungsgemeinschaft, C. W. M. is the recipient of a Cancer Research Institute/Chase Manhattan Bank fellowship, and D. H. B. is a recipient of a Research Fellowship from the Howard Hughes Medical Institute. F. A. L. was supported by the Institut Pasteur. This work was supported by National Institutes of Health grant RO-1AI35021 to D. H. R.
Funding AgencyGrant Number
Deutsche Forschungsgemeinschaft (DFG)UNSPECIFIED
Cancer Research InstituteUNSPECIFIED
Chase Manhattan BankUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Institut PasteurUNSPECIFIED
Issue or Number:1
Record Number:CaltechAUTHORS:20170405-154817252
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Official Citation:Thomas Hanke, Hisao Takizawa, Christopher W McMahon, Dirk H Busch, Eric G Pamer, Joseph D Miller, John D Altman, Yang Liu, Dragana Cado, Francois A Lemonnier, Pamela J Bjorkman, David H Raulet, Direct Assessment of MHC Class I Binding by Seven Ly49 Inhibitory NK Cell Receptors, Immunity, Volume 11, Issue 1, 1 July 1999, Pages 67-77, ISSN 1074-7613, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:75755
Deposited By: Tony Diaz
Deposited On:05 Apr 2017 23:03
Last Modified:15 Nov 2021 16:36

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