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Cytoarchitectonic mapping by microdensitometry

Hudspeth, A. J. and Ruark, J. E. and Kelly, J. P. (1976) Cytoarchitectonic mapping by microdensitometry. Proceedings of the National Academy of Sciences of the United States of America, 73 (8). pp. 2928-2931. ISSN 0027-8424.

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Functional differences among various portions of the cerebral cortex are often correlated with differing cortical layering patterns. Convenient, accurate techniques for scoring layering should therefore prove useful in electrophysiological as well as anatomical investigations. We report the application of a computer-controlled scanning microdensitometer as a means of rapid measurement of optical densities in histological sections of monkey visual cortices, areas 17 and 18. The technique readily permits recognition of the previously defined cortical layers and suggests that still finer consistent layering patterns exist; it provides objective "fingerprints" of cortical regions which facilitate comparisons of structure from area to area and from animal to animal. The procedure should serve also to score the positions of autoradiographic grains, degenerating axonal terminals, and other labeled structures, and to allow the comparison of preparations stained by various techniques.

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Additional Information:© 1976 by the National Academy of Sciences. Communicated by James Olds, May 24, 1976. The authors thank Dr. R.M. Stroud for the use of the microdensitometer and computer facilities and for comments on the manuscript, Drs. T.N. Wiesel and D.H. Hubel for the use of some of the histological material, and Dr. M. Ross for assistance in computer programming. This project was supported by a grant from the California Institute of Technology President's Venture Fund (A.J.H.), by National Science Foundation Grant BMS75-04105 to Dr. R.M. Stroud (J.E.R.), and by grants from The Spencer Foundation and The Hixon Fund (J.P.K.).
Subject Keywords:visual cortex; monkey; computer
Issue or Number:8
Record Number:CaltechAUTHORS:HUDpnas76
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7577
Deposited By: Tony Diaz
Deposited On:06 Mar 2007
Last Modified:02 Oct 2019 23:43

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