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Expression and Crystallization of a Soluble Form of Drosophila Fasciclin III

Strong, Roland K. and Vaughn, Daniel E. and Bjorkman, Pamela J. and Snow, Peter M. (1994) Expression and Crystallization of a Soluble Form of Drosophila Fasciclin III. Journal of Molecular Biology, 241 (3). pp. 483-487. ISSN 0022-2836.

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A truncated form of Drosophila fasciclin III has bee engineered by site-directed mutagenesis. Secreted fasciclin III is expressed at 35 to 40 mg/l in insert cells with baculovirus carrying the recombinant gene. Single crystals of purified soluble fasciclin III have been grown by vapor diffusion versus polyethylene glycol 8000/sodium citrate at low pH. The space group is P6_122 or its enantiomorph P6_522, with unit cell dimensions ɑ = b = 140 Å, c = 260 Å. Cryo-preserved crystals diffract to reciprocal lattice spacing beyond 3·0 Å.

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Bjorkman, Pamela J.0000-0002-2277-3990
Additional Information:© 1994 Academic Press. This work was supported by a Basil O'Connor Starter Scholar Award (P.M.S.). the National Institute of Health (R29 NS28699 to P.M.S.) and the Howard Hughes Medical Institute (P.J.B.). P.J.B. was a Pew Scholar and held a Young Investigator award from the Cancer Research Institute during part of the work. R.K.S. was a post-doctoral fellow of the American Cancer Society, D.E.V. is supported by an NIH pre-doctoral training grant. We thank M.D. Summers for the gift of a baculovirus starter kit, Michael Blum and Leemor Joshua-Tor, who provided instruction on the cryo-preservation of maromolecular crystals, and Michael Soltis (SSRL.). Leemor Joshua-Tor and Michael Stowell for assistance with data collection at SSRL.
Funding AgencyGrant Number
NIHR29 NS28699
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Cancer Research InstituteUNSPECIFIED
NIH Predoctoral FellowshipUNSPECIFIED
Pew Charitable TrustUNSPECIFIED
American Cancer SocietyUNSPECIFIED
Issue or Number:3
Record Number:CaltechAUTHORS:20170406-085029763
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Official Citation:Roland K. Strong, Daniel E. Vaughn, Pamela J. Bjorkman, Peter M. Show, Expression and Crystallization of a Soluble Form of Drosophila Fasciclin III, Journal of Molecular Biology, Volume 241, Issue 3, 1994, Pages 483-487, ISSN 0022-2836, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:75785
Deposited By: Ruth Sustaita
Deposited On:06 Apr 2017 16:36
Last Modified:03 Oct 2019 16:54

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