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Expression and Crystallization of a Soluble Form of Drosophila Fasciclin III

Strong, Roland K. and Vaughn, Daniel E. and Bjorkman, Pamela J. and Snow, Peter M. (1994) Expression and Crystallization of a Soluble Form of Drosophila Fasciclin III. Journal of Molecular Biology, 241 (3). pp. 483-487. ISSN 0022-2836. https://resolver.caltech.edu/CaltechAUTHORS:20170406-085029763

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Abstract

A truncated form of Drosophila fasciclin III has bee engineered by site-directed mutagenesis. Secreted fasciclin III is expressed at 35 to 40 mg/l in insert cells with baculovirus carrying the recombinant gene. Single crystals of purified soluble fasciclin III have been grown by vapor diffusion versus polyethylene glycol 8000/sodium citrate at low pH. The space group is P6_122 or its enantiomorph P6_522, with unit cell dimensions ɑ = b = 140 Å, c = 260 Å. Cryo-preserved crystals diffract to reciprocal lattice spacing beyond 3·0 Å.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1006/jmbi.1994.1523DOIArticle
http://www.sciencedirect.com/science/article/pii/S0022283684715233PublisherArticle
ORCID:
AuthorORCID
Bjorkman, Pamela J.0000-0002-2277-3990
Additional Information:© 1994 Academic Press. This work was supported by a Basil O'Connor Starter Scholar Award (P.M.S.). the National Institute of Health (R29 NS28699 to P.M.S.) and the Howard Hughes Medical Institute (P.J.B.). P.J.B. was a Pew Scholar and held a Young Investigator award from the Cancer Research Institute during part of the work. R.K.S. was a post-doctoral fellow of the American Cancer Society, D.E.V. is supported by an NIH pre-doctoral training grant. We thank M.D. Summers for the gift of a baculovirus starter kit, Michael Blum and Leemor Joshua-Tor, who provided instruction on the cryo-preservation of maromolecular crystals, and Michael Soltis (SSRL.). Leemor Joshua-Tor and Michael Stowell for assistance with data collection at SSRL.
Funders:
Funding AgencyGrant Number
NIHR29 NS28699
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Cancer Research InstituteUNSPECIFIED
NIH Predoctoral FellowshipUNSPECIFIED
Pew Charitable TrustUNSPECIFIED
American Cancer SocietyUNSPECIFIED
Issue or Number:3
Record Number:CaltechAUTHORS:20170406-085029763
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170406-085029763
Official Citation:Roland K. Strong, Daniel E. Vaughn, Pamela J. Bjorkman, Peter M. Show, Expression and Crystallization of a Soluble Form of Drosophila Fasciclin III, Journal of Molecular Biology, Volume 241, Issue 3, 1994, Pages 483-487, ISSN 0022-2836, http://dx.doi.org/10.1006/jmbi.1994.1523. (http://www.sciencedirect.com/science/article/pii/S0022283684715233)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:75785
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:06 Apr 2017 16:36
Last Modified:03 Oct 2019 16:54

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