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BIAcore: a microchip-based system for analyzing the formation of macromolecular complexes

Raghavan, Malini and Bjorkman, Pamela J. (1995) BIAcore: a microchip-based system for analyzing the formation of macromolecular complexes. Structure, 3 (4). pp. 331-333. ISSN 09692126. doi:10.1016/S0969-2126(01)00164-2.. https://resolver.caltech.edu/CaltechAUTHORS:20170406-094024240

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Abstract

The formation of macromolecular complexes, for example the complex between an antibody and an antigen, is essential to many biological processes. In order to gain a better insight into the biological processes involved, the formation of complexes is often measured and their interaction kinetics and affinities analyzed. Considerable effort has been expended in developing techniques to measure the formation of complexes, the most promising being electronic microchip-based biosensors. Such analytical systems have come into increasing use recently for the characterization of interactions between biological macromolecules and ligands. One such commercially available system, called BIAcore (Pharmacia Biosensor, Uppsala, Sweden), uses biosensor-based technology and offers a method for rapid analysis of interaction kinetics and affinities. Such systems have the advantage of replacing tedious binding assays utilizing radiolabelled or fluorescent components and potentially unstable cell lines, and can be used where biological activity assays are not available.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0969-2126(01)00164-2.DOIArticle
http://www.sciencedirect.com/science/article/pii/S0969212601001642PublisherArticle
ORCID:
AuthorORCID
Bjorkman, Pamela J.0000-0002-2277-3990
Additional Information:© 1995 Elsevier Science Ltd. We thank Shirley Demer of Pharmacia Biosensor for technical advice and critical reading of this manuscript. Some of the work described here was supported by the Howard Hughes Medical Institute (PJB) and by a post-doctoral fellowship from the Cancer Research Institute (MR).
Funders:
Funding AgencyGrant Number
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Cancer Research InstituteUNSPECIFIED
Issue or Number:4
DOI:10.1016/S0969-2126(01)00164-2.
Record Number:CaltechAUTHORS:20170406-094024240
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170406-094024240
Official Citation:Malini Raghavan, Pamela J Bjorkman, BIAcore: a microchip-based system for analyzing the formation of macromolecular complexes, Structure, Volume 3, Issue 4, April 1995, Pages 331-333, ISSN 0969-2126, http://dx.doi.org/10.1016/S0969-2126(01)00164-2. (http://www.sciencedirect.com/science/article/pii/S0969212601001642)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:75791
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:06 Apr 2017 16:48
Last Modified:15 Nov 2021 16:36

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