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Silicon chip-based patch-clamp electrodes integrated with PDMS microfluidics

Pantoja, Rigo and Nagarah, John M. and Starace, Dorine M. and Melosh, Nicholas A. and Blunck, Rikard and Bezanilla, Francisco and Heath, James R. (2004) Silicon chip-based patch-clamp electrodes integrated with PDMS microfluidics. Biosensors and Bioelectronics, 20 (3). pp. 509-517. ISSN 0956-5663.

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We report on a silicon wafer-based device that can be used for recording macroscopic ion channel protein activities across a diverse group of cell-types. Gigaohm seals were achieved for CHO-K1 and RIN m5F cells, and both cell-attached and whole-cell mode configurations were also demonstrated. Two distinct intrinsic potassium ion channels were recorded in whole-cell mode for HIT-T15 and RAW 264.7 cells. Polydimethylsiloxane (PDMS) microfluidics were also coupled with the micromachined silicon chips in order to demonstrate that a single cell could be selectively directed to a micropore, and membrane protein currents could subsequently be recorded. These silicon chip-based devices have significant advantages over traditional micropipette approaches, and may serve as combinatorial tools for investigating membrane biophysics, pharmaceutical screening, and other bio-sensing tasks.

Item Type:Article
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Blunck, Rikard0000-0003-4484-2907
Heath, James R.0000-0001-5356-4385
Additional Information:© 2004 Elsevier. Received 7 October 2003, Revised 15 February 2004, Accepted 25 February 2004, Available online 6 May 2004. This work was funded by the W.M. Keck Foundation, the Semiconductor Research Corporation, the NASA-funded CMISE center, and NIH grant Gm30376. We would like to thank B. Ribalet, and A. Aderem for supplying the RIN m5F, and RAW 264.7 cells, respectfully. We also acknowledge the UCLA Nanolab for assistance and advice in device fabrication.
Subject Keywords:Silicon microchip; BioMEMS; Biosensor; Patch-clamp; Electrophysiology; Biomembranes
Issue or Number:3
Record Number:CaltechAUTHORS:20170408-135725447
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Official Citation:Rigo Pantoja, John M. Nagarah, Dorine M. Starace, Nicholas A. Melosh, Rikard Blunck, Francisco Bezanilla, James R. Heath, Silicon chip-based patch-clamp electrodes integrated with PDMS microfluidics, Biosensors and Bioelectronics, Volume 20, Issue 3, 15 October 2004, Pages 509-517, ISSN 0956-5663, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:75893
Deposited By: 1Science Import
Deposited On:14 Apr 2017 19:16
Last Modified:03 Oct 2019 16:55

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