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Vesicular stomatitis virus glycoprotein is necessary for H-2-restricted lysis of infected cells by cytotoxic T lymphocytes

Hale, Arthur H. and Witte, Owen N. and Baltimore, David and Eisen, Herman N. (1978) Vesicular stomatitis virus glycoprotein is necessary for H-2-restricted lysis of infected cells by cytotoxic T lymphocytes. Proceedings of the National Academy of Sciences of the United States of America, 75 (2). pp. 970-974. ISSN 0027-8424. PMCID PMC411381.

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Vesicular stomatitis virus (VSV) elicited cytotoxic thymus-derived lymphocytes (CTLs) in mice of the BALB/c and three congenic strains (BALB.b, BALB.k, BALB.HTG). CTL lysis of VSV-infected fibroblasts from the four strains was restricted by the target cells' major histocompatibility complex (H-2). Target cells were also infected with two temperature-sensitive mutants of VSV, tsM and tsG in which, respectively, the viral matrix protein and glycoprotein are not expressed at 39 degrees (restrictive temperature) on the infected cell's surface membrane. At the restrictive temperature, cells infected with wild-type VSV or tsM were lysed by CTLs, but cells infected with tsG were not. The requirement for the glycoprotein on the target cell was also evident from the ability of antisera to the glycoprotein to block completely CTL lysis of VSV-infected cells.

Item Type:Article
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URLURL TypeDescription CentralArticle
Witte, Owen N.0000-0003-4461-4533
Baltimore, David0000-0001-8723-8190
Additional Information:© 1978 by the National Academy of Sciences. Contributed by Herman N. Eisen, December 5, 1977. We thank E. A. Boyse, R. J. Graff, and F. Lilly for the breeders used to establish colonies of BALB/cAnN, BALB.b, BALB.k, and BALB.HTG mice. D.B. is a Research Professor of the American Cancer Society; A.H.H. is the recipient of Postdoctoral Fellowship Award 5 F32 CA05685 from the National Cancer Institute; O.N.W. is a fellow of the Helen Hay Whitney Foundation. This work was supported by Research Grant VC-41 from the American Cancer Society and by Research Grant CA-15472 and Center Grant CA-14051 from the National Cancer Institute, Department of Health, Education, and Welfare. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact.
Funding AgencyGrant Number
American Cancer SocietyVC-41
NIH Postdoctoral Fellowship5 F32 CA05685
Helen Hay Whitney FoundationUNSPECIFIED
Subject Keywords:antigen recognition; viral immunity; temperature-sensitive mutants; cell surface antigens
Issue or Number:2
PubMed Central ID:PMC411381
Record Number:CaltechAUTHORS:HALpnas78
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7608
Deposited By: Tony Diaz
Deposited On:13 Mar 2007
Last Modified:02 Oct 2019 23:43

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