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15N NMR study of a mixture of uniformly labeled tRNAs

Gonnella, Nina C. and Birdseye, Theresa R. and Nee, Michael and Roberts, John D. (1982) 15N NMR study of a mixture of uniformly labeled tRNAs. Proceedings of the National Academy of Sciences of the United States of America, 79 (16). pp. 4834-4837. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:GONpnas82

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Abstract

15N NMR spectra were taken of 15N-enriched tRNA extracted from bakers yeast; ammonium sulfate was used as a nitrogen source. The increase in the degree of denaturation of tRNA, which occurs with increase in temperature from 30 degrees C to 70 degrees C, resulted in no large changes in 15N chemical shifts at acidic and neutral pH but quite pronounced changes in proton-15N nuclear Overhauser effects.


Item Type:Article
Additional Information:© 1982 by the National Academy of Sciences. Contributed by John D. Roberts, April 9, 1982. We are pleased to acknowledge use of the Southern California Regional NMR Center facilities (Bruker MW-500 spectrometer) supported by National Science Foundation Grant CHE79-16324. This research was supported by the U.S. Public Health Service (Grant GM-11072 from the Division of General Medical Sciences) and by the National Science Foundation. This constitutes Contribution 6617 from the Gates and Crellin Laboratories of Chemistry.
Subject Keywords:15N chemical shift; nuclear Overhauser effects; nucleotide-base nitrogen resonances; denaturation of tRNA
Record Number:CaltechAUTHORS:GONpnas82
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:GONpnas82
Alternative URL:http://dx.doi.org/10.1073/pnas.79.16.4834
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:7614
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:14 Mar 2007
Last Modified:14 Nov 2014 19:19

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