Joern, John M and Meinhold, Peter and Arnold, Frances H. (2002) Analysis of shuffled gene libraries. Journal of Molecular Biology, 316 (3). pp. 643-656. ISSN 0022-2836. doi:10.1006/jmbi.2001.5349. https://resolver.caltech.edu/CaltechAUTHORS:20170408-161123738
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Abstract
In vitro recombination of homologous genes (family shuffling) has been proposed as an effective search strategy for laboratory evolution of genes and proteins. Few data are available, however, on the composition of shuffled gene libraries, from which one could assess the efficiency of recombination and optimize protocols. Here, probe hybridization is used in a macroarray format to analyze chimeric DNA libraries created by DNA shuffling. Characterization of hundreds of shuffled genes encoding dioxygenases has elucidated important biases in the shuffling reaction. As expected, crossovers are favored in regions of high sequence identity. A sequence-based model of homologous recombination that captures this observed bias was formulated using the experimental results. The chimeric genes were found to show biases in the incorporation of sequences from certain parents, even before selection. Statistically different patterns of parental incorporation in genes expressing functional proteins can help to identify key sequence-function relationships.
Item Type: | Article | ||||||
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Additional Information: | © 2002 Elsevier. Received 5 October 2001, Revised 14 December 2001, Accepted 14 December 2001, Available online 5 March 2002. The authors thank Jonathan Silberg, Christopher Voigt and Lillian Pierce for their comments on the manuscript. This work was supported by the U. S. National Science Foundation (BES#9981770) and by Maxygen, Inc. | ||||||
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Subject Keywords: | directed evolutionrecombinationfamily shufflingStEPrandom chimeragenesis | ||||||
Issue or Number: | 3 | ||||||
DOI: | 10.1006/jmbi.2001.5349 | ||||||
Record Number: | CaltechAUTHORS:20170408-161123738 | ||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20170408-161123738 | ||||||
Official Citation: | John M. Joern, Peter Meinhold, Frances H. Arnold, Analysis of shuffled gene libraries , Journal of Molecular Biology, Volume 316, Issue 3, 2002, Pages 643-656, ISSN 0022-2836, http://dx.doi.org/10.1006/jmbi.2001.5349. (http://www.sciencedirect.com/science/article/pii/S0022283601953495) | ||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||
ID Code: | 76143 | ||||||
Collection: | CaltechAUTHORS | ||||||
Deposited By: | 1Science Import | ||||||
Deposited On: | 22 Jun 2017 20:38 | ||||||
Last Modified: | 15 Nov 2021 16:57 |
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