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The Transcription Factor WIN1/SHN1 Regulates Cutin Biosynthesis in Arabidopsis thaliana

Kannangara, Rubini and Branigan, Caroline and Liu, Yan and Penfield, Teresa and Rao, Vijaya and Mouille, Grégory and Höfte, Herman and Pauly, Markus and Riechmann, José Luis and Broun, Pierre (2007) The Transcription Factor WIN1/SHN1 Regulates Cutin Biosynthesis in Arabidopsis thaliana. Plant Cell, 19 (4). pp. 1278-1294. ISSN 1040-4651. PMCID PMC1913754. https://resolver.caltech.edu/CaltechAUTHORS:20170408-172815604

[img] Image (TIFF) (Supplemental Figure 1 - Monosaccharide composition of shoot and root cell walls in 35S:WIN1 overexpressors) - Supplemental Material
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[img] Image (TIFF) (Supplemental Figure 2 - Dendogram obtained by hierarchical cluster analysis of 42 Arabidopsis lines based on FT-IR spectra sampled from dark-grown hypocotyls) - Supplemental Material
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[img] Image (TIFF) (Supplemental Figure 3 - Composition of leaf acyl-CoAs and membrane fatty acids in 35S:WIN1 plants) - Supplemental Material
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[img] Image (TIFF) (Supplemental Figure 4 - Phylogenetic analysis of genes related to DEX-induced GPAT4 and HTH-like) - Supplemental Material
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[img] MS Word (Supplemental Figure 5 - Alignment of the predicted amino acid sequences of proteins related to HTH) - Supplemental Material
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[img] MS Word (Supplemental Figure 6 - Alignment of the predicted amino acid sequences of proteins related to GPAT4) - Supplemental Material
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[img] MS Excel (Supplemental Table 1 - Short-term regulation of gene expression by WIN1-HA: output of different statistical analyses of microarray data) - Supplemental Material
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[img] MS Excel (Supplemental Table 2 - Promoter motifs in genes that were significantly up-regulated after WIN1-HA induction by DEX in pOp6:WIN-HA plants) - Supplemental Material
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Abstract

he composition and permeability of the cuticle has a large influence on its ability to protect the plant against various forms of biotic and abiotic stress. WAX INDUCER1 (WIN1) and related transcription factors have recently been shown to trigger wax production, enhance drought tolerance, and modulate cuticular permeability when overexpressed in Arabidopsis thaliana. We found that WIN1 influences the composition of cutin, a polyester that forms the backbone of the cuticle. WIN1 overexpression induces compositional changes and an overall increase in cutin production in vegetative and reproductive organs, while its downregulation has the opposite effect. Changes in cutin composition are preceded by the rapid and coordinated induction of several genes known or likely to be involved in cutin biosynthesis. This transcriptional response is followed after a delay by the induction of genes associated with wax biosynthesis, suggesting that the regulation of cutin and wax production by WIN1 is a two-step process. We demonstrate that at least one of the cutin pathway genes, which encodes long-chain acyl-CoA synthetase LACS2, is likely to be directly targeted by WIN1. Overall, our results suggest that WIN1 modulates cuticle permeability in Arabidopsis by regulating genes encoding cutin pathway enzymes.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1105/tpc.106.047076DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1913754/PubMed CentralArticle
ORCID:
AuthorORCID
Liu, Yan0000-0002-5837-4908
Additional Information:© 2007 The American Society of Plant Biologists. Received September 4, 2006; revised March 14, 2007; accepted April 3, 2007; published April 20, 2007. We thank Toshiro Ito for providing the pGTI0242 vector, Christiane Nawrath for her assistance with mass spectral analysis, Naveed Aziz for his technical support with microarray hybridizations and data analysis, and Uzma Urooj for her technical assistance with this project. This work was supported by Grant P20283 from the Biotechnology and Biological Sciences Research Council (UK). The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantcell.org) is: Pierre Broun (pbroun@york.ac.uk).
Group:Millard and Muriel Jacobs Genetics and Genomics Laboratory
Funders:
Funding AgencyGrant Number
Biotechnology and Biological Sciences Research Council (BBSRC)P20283
Issue or Number:4
PubMed Central ID:PMC1913754
Record Number:CaltechAUTHORS:20170408-172815604
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170408-172815604
Official Citation:The Transcription Factor WIN1/SHN1 Regulates Cutin Biosynthesis in Arabidopsis thaliana Rubini Kannangara, Caroline Branigan, Yan Liu, Teresa Penfield, Vijaya Rao, Grégory Mouille, Herman Höfte, Markus Pauly, José Luis Riechmann, Pierre Broun The Plant Cell Apr 2007, 19 (4) 1278-1294; DOI: 10.1105/tpc.106.047076
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:76354
Collection:CaltechAUTHORS
Deposited By: 1Science Import
Deposited On:29 Mar 2018 23:05
Last Modified:09 Mar 2020 13:19

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