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Tissue-Specific Regulation of the LIM Homeobox Gene lin-11 during Development of the Caenorhabditis elegans Egg-Laying System

Gupta, Bhagwati P. and Sternberg, Paul W. (2002) Tissue-Specific Regulation of the LIM Homeobox Gene lin-11 during Development of the Caenorhabditis elegans Egg-Laying System. Developmental Biology, 247 (1). pp. 102-115. ISSN 0012-1606. doi:10.1006/dbio.2002.0688. https://resolver.caltech.edu/CaltechAUTHORS:20170408-192830845

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Abstract

The egg-laying system of Caenorhabditis elegans hermaphrodites requires development of the vulva and its precise connection with the uterus. This process is regulated by LET-23-mediated epidermal growth factor signaling and LIN-12-mediated lateral signaling pathways. Among the nuclear factors that act downstream of these pathways, the LIM homeobox gene lin-11 plays a major role. lin-11 mutant animals are egg-laying defective because of the abnormalities in vulval lineage and uterine seam–cell formation. However, the mechanisms providing specificity to lin-11 function are not understood. Here, we examine the regulation of lin-11 during development of the egg-laying system. Our results demonstrate that the tissue-specific expression of lin-11 is controlled by two distinct regulatory elements that function as independent modules and together specify a wild-type egg-laying system. A uterine π lineage module depends on the LIN-12/Notch signaling, while a vulval module depends on the LIN-17-mediated Wnt signaling. These results provide a unique example of the tissue-specific regulation of a LIM homeobox gene by two evolutionarily conserved signaling pathways. Finally, we provide evidence that the regulation of lin-11 by LIN-12/Notch signaling is directly mediated by the Su(H)/CBF1 family member LAG-1.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1006/dbio.2002.0688DOIArticle
ORCID:
AuthorORCID
Sternberg, Paul W.0000-0002-7699-0173
Additional Information:© 2002 Elsevier. Received 7 January 2002, Revised 3 April 2002, Accepted 8 April 2002, Available online 21 June 2002. We thank S. Cameron for the nIs96 strain and Plin-11gfp plasmid, Y. Kohara for the lin-11 cDNA clone, J. Kimble for the lag-1 plasmid, and Fire Lab for GFP vectors. Some of the strains were provided by the C. elegans Genetics Center. We also thank B. Hwang for advice on the RNAi experiment and R. Garcia, D. Sherwood, and B. Hwang for comments on the manuscript. B.P.G. was supported by a long-term HFSP fellowship. P.W.S. is an Investigator and B.P.G. an Associate with the Howard Hughes Medical Institute, which funded this research.
Funders:
Funding AgencyGrant Number
Human Frontier Science ProgramUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Subject Keywords:C. elegans; egg-laying; vulva; uterus; LIN-11; Wnt; Notch
Issue or Number:1
DOI:10.1006/dbio.2002.0688
Record Number:CaltechAUTHORS:20170408-192830845
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20170408-192830845
Official Citation:Bhagwati P. Gupta, Paul W. Sternberg, Tissue-Specific Regulation of the LIM Homeobox Gene lin-11 during Development of the Caenorhabditis elegans Egg-Laying System, Developmental Biology, Volume 247, Issue 1, 1 July 2002, Pages 102-115, ISSN 0012-1606, https://doi.org/10.1006/dbio.2002.0688. (http://www.sciencedirect.com/science/article/pii/S0012160602906883)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:76364
Collection:CaltechAUTHORS
Deposited By: 1Science Import
Deposited On:22 Jun 2017 21:23
Last Modified:15 Nov 2021 16:59

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